Gotu Kola (Centella Asiatica) extract enhances phosphorylation of cyclic AMP response element binding protein in neuroblastoma cells expressing amyloid beta peptide.
Gotu Kola (Centella Asiatica) extract enhances phosphorylation of cyclic AMP response element binding protein in neuroblastoma cells expressing amyloid beta peptide.
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DOI：
10.3233/jad-2008-13311
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Yanan Xu;Z. Cao;I. Khan;Yuan Luo
中科院分区：
文献类型：
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作者：
Yanan Xu;Z. Cao;I. Khan;Yuan Luo
阿尔茨海默病（AD）是一种进行性神经退行性疾病，表现为认知缺陷和记忆障碍。从Gotu Kola（积雪草亚洲）的叶子提取物已被用作一种替代药物，以改善记忆在印度阿育吠陀医学系统很长一段时间。虽然有研究发现其具有改善AD大鼠模型认知功能障碍的作用以及对海马区神经元树突的刺激作用，但其对神经保护的分子机制仍有待阐明。在这项研究中，我们报告了在表达淀粉样蛋白β 1-42 （Abeta）的神经母细胞瘤细胞系和大鼠胚胎皮质原代细胞培养中，环AMP反应元件结合蛋白（CREB）的磷酸化都增强。此外，研究了两个主要单一组分对CREB磷酸化增强的贡献。此外，在本研究中，抑制剂的应用揭示了ERK/RSK信号通路可能介导了Gotu Kola提取物的这种作用。本研究首次为枸杞提取物增强记忆提供了可能的分子机制。
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that shows cognitive deficits and memory impairment. Extract from the leaves of Gotu Kola (Centella Asiatica) have been used as an alternative medicine for memory improvement in Indian Ayurvedic system of medicine for a long time. Although several studies have revealed its effect in ameliorating the cognitive impairment in rat models of AD and stimulating property on neuronal dendrites of hippocampal region, the molecular mechanism of Gotu Kola on neuroprotection still remains to be elucidated. In this study, we report that phosphorylation of cyclic AMP response element binding protein (CREB) is enhanced in both a neuroblastoma cell line expressing amyloid beta 1-42 (Abeta) and in rat embryonic cortical primary cell culture. In addition, the contribution of two major single components to the enhanced CREB phosphorylatioin was examined. Furthermore, inhibitors were applied in this study revealing that ERK/RSK signaling pathway might mediate this effect of Gotu Kola extract. Taken together, we provide a possible molecular mechanism for memory enhancing property of Gotu Kola extract for the first time.