BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment of ligature-induced periodontitis in dogs
BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment of ligature-induced periodontitis in dogs
复制标题
DOI：
10.1002/jper.18-0070
复制
发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Kurihara, Hidemi
中科院分区：
文献类型：
--
作者：
Sasaki, Shinya;Takeda, Katsuhiro;Kurihara, Hidemi
背景近年来，脑源性神经营养因子（BDNF）/高分子量透明质酸（HMW-HA）复合物与皮瓣手术联合应用被证明能促进牙周组织再生。本研究的目的是评价局部龈下应用BDNF/HMW-HA复合物对狗结扎性牙周炎的影响。方法将实验犬分为4组：对照组、单纯SRP组、SRP+HMW-HA组和SRP + BDNF（500 μ g/ml）/ HMW-HA复合物组。使用注射器将HMW-HA或BDNF/HMW-HA复合物局部应用于牙周袋，而无需手术。治疗后两周，记录临床参数（牙龈指数，临床附着水平，牙周袋深度和探诊出血），并从麻醉动物中收集标本进行组织学分析。结果SRP+BDNF/HMW-HA治疗组各项临床指标均较其他治疗组明显改善。组织学分析显示，与其他治疗组相比，SRP+BDNF/HMW-HA组对上皮组织顶端迁移的抑制更大，炎症细胞浸润更轻。SRP+BDNF/HMW-HA组牙骨质和牙槽骨再生，胶原纤维嵌入其中。结论BDNF/HMW-HA复合物作为牙周病非手术治疗的辅助药物，具有减轻过度炎症的作用。需要进一步的研究来阐明BDNF/HMW-HA复合物在非手术环境中对牙周组织再生的作用。
Background Recently, brain-derived neurotrophic factor (BDNF)/high molecular weight hyaluronic acid (HMW-HA) complex with flap surgery has been shown to promote periodontal tissue regeneration. The objective of this study was to evaluate the effects of local subgingival application of BDNF/HMW-HA complex adjunctive to scaling and root planning (SRP) on ligature-induced periodontitis in dogs. Methods The dogs were divided into four treatment groups: no treatment (control), SRP alone, SRP followed by local application of HMW-HA (SRP+HMW-HA), and SRP followed by local application of BDNF (500 mu g/ml)/ HMW-HA complex (SRP+BDNF/HMW-HA). HMW-HA or BDNF/HMW-HA complex was topically applied to periodontal pockets using a syringe without surgery. Two weeks after treatment, clinical parameters (gingival index, clinical attachment level, periodontal pocket depth and bleeding on probing) were recorded and specimens were collected from anesthetized animals for histological analysis. Results The SRP+BDNF/HMW-HA group showed significant improvement in all clinical parameters compared to other treatment groups. Histologic analysis showed greater suppression of apical migration of epithelial tissue and milder inflammatory cell infiltration in the SRP+BDNF/HMW-HA group than in the other treatment groups. Furthermore, new cementum and alveolar bone were regenerated, and collagen fibers were inserted into them in the SRP+BDNF/HMW-HA group. Conclusion BDNF/HMW-HA complex as an adjunct to nonsurgical periodontal treatment has the potential to reduce excess inflammation. Further investigation will be needed to clarify periodontal tissue regenerative effects of BDNF/HMW-HA complex in a nonsurgical setting.