Interleukin-1α expression in capillaries and major histocompatibility complex class I expression in type II muscle fibers from polymyositis and dermatomyositis patients -: Important pathogenic features independent of inflammatory cell clusters in muscle tissue
Interleukin-1α expression in capillaries and major histocompatibility complex class I expression in type II muscle fibers from polymyositis and dermatomyositis patients -: Important pathogenic features independent of inflammatory cell clusters in muscle tissue
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DOI：
10.1002/art.10140
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发表时间：
2002-04-01
影响因子：
--
通讯作者：
Lundberg, IE
中科院分区：
文献类型：
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作者：
Englund, P;Nennesmo, I;Lundberg, IE
目标。通过对多发性肌炎（PM）和皮肌炎（DM）患者肌肉组织的分析，提出内皮细胞和/或肌纤维是疾病过程中主要目标的假设。我们纳入了具有典型肌炎的实验室体征和临床症状的患者，但在他们的肌肉活检样本中没有检测到炎症细胞簇的浸润。应用免疫组织化学技术鉴定肌肉组织中的CD3、CD68、淋巴细胞功能相关抗原1 α、CD11b、极晚活化抗原4、内皮素4、白细胞介素-la （il -1 α）、细胞间粘附分子1、血管细胞粘附分子1、IgG、IgM、IgA和hi -a /B/C。通过atp酶染色确定纤维类型。结果。患者毛细血管内皮细胞中il -1 α的表达高于对照组，肌纤维中I类主要组织相容性复合体（MHC）的表达显著增加。我们还观察到I类MHC的表达主要局限于II型肌纤维。我们的研究结果表明，血管和肌肉纤维的特定分子变化与邻近的炎症浸润无关，因此可能是肌炎发展的主要事件。此外，il -1 α和I类MHC分子可能对PM和DM患者临床症状的发展都很重要。
Objective. To address the hypothesis that endothelial cells and/or muscle fibers are primary targets in the disease process by analysis of muscle tissue from patients with polymyositis (PM) and dermatomyositis (DM).Methods. We included patients with laboratory signs and clinical symptoms typical of myositis, but without detectable infiltration of clusters of inflammatory cells in their muscle biopsy samples. An immunohistochemical technique was applied to identify CD3, CD68, lymphocyte function-associated antigen 1alpha, CD11b, very late activation antigen 4, endothelium 4, interleukin-la (IL-1alpha), intercellular adhesion molecule 1, vascular cell adhesion molecule 1, IgG, IgM, IgA, and HI-A-A/B/C in muscle tissue. Fiber type was defined by ATPase staining. Results. IL-1alpha expression was detected in endothelial cells of capillaries to a greater extent in patients than in controls, and class I major histocompatibility complex (MHC) expression was significantly increased in muscle fibers. We also observed that class I MHC expression was mainly confined to type II muscle fibers.Conclusion. Our findings imply that defined molecular changes of blood vessels and muscle fibers are both independent of adjacent inflammatory infiltrates and could thus be primary events in the development of myositis. Moreover, both IL-1alpha and class I MHC molecules might be important for the development of clinical symptoms in PM and DM patients.