Alkaline transfer of DNA to plastic membrane.
Alkaline transfer of DNA to plastic membrane.
复制标题
DNA 碱转移至塑料膜。
DOI：
10.1016/0006-291x(84)90480-7
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发表时间：
1984
影响因子：
3.1
通讯作者：
P. Qasba
中科院分区：
文献类型：
--
作者：
P. Chomczyński;P. Qasba
在碱性条件下，DNA与Gene Screen Plus膜形成稳定的复合物，并以此为基础，建立了DNA从琼脂糖凝胶上碱转移到Gene Screen Plus膜上的方法。该程序需要将0.4 M NaOH用于DNA变性和DNA转移步骤。与中性pH下的DNA转移标准方法相比，碱性转移提供了更高的杂交效率并简化了转移程序。此外，它可用于从转移的DNA中去除RNA污染。
DNA forms a stable complex with Gene Screen Plus membrane at alkaline pH. Based on this, a method of alkaline transfer of DNA from agarose gel to Gene Screen Plus membrane was elaborated. The procedure entails the use of 0.4 M NaOH for both, the DNA denaturation and DNA transfer steps. The alkaline transfer offers a higher hybridization efficiency and simplifies the transfer procedure as compared with the standard method of DNA transfer at neutral pH. In addition, it can be used to remove RNA contamination from the transferred DNA.