DNA-REPAIR RATES MAPPED ALONG THE HUMAN PGK1 GENE AT NUCLEOTIDE RESOLUTION
DNA-REPAIR RATES MAPPED ALONG THE HUMAN PGK1 GENE AT NUCLEOTIDE RESOLUTION
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DOI：
10.1126/science.8128226
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发表时间：
1994-03-11
期刊：
影响因子：
56.9
通讯作者：
HOLMQUIST, GP
中科院分区：
文献类型：
--
作者：
GAO, SW;DROUIN, R;HOLMQUIST, GP
在核苷酸分辨下，研究了紫外光诱导的DNA损伤--环丁烷嘧啶二聚体(CPDS)的修复。用紫外线照射人成纤维细胞，并允许其修复。DNA在CPD上被酶切，并通过连接介导的聚合酶链式反应定位沿着PGK1基因启动子和外显子1的诱导断裂。根据核苷酸位置的不同，非转录链的修复率差异高达15倍。转录链的优先修复开始于转录起始点的下游，但最明显的开始于外显子1的+140核苷酸。启动子包含两个缓慢修复的区域，这两个区域与两个转录因子结合位点重合。
The repair of cyclobutane pyrimidine dimers (CPDs), DNA lesions induced by ultraviolet light, was studied at nucleotide resolution. Human fibroblasts were irradiated with ultraviolet light and allowed to repair. The DNA was enzymatically cleaved at the CPDs, and the induced breaks along the promoter and exon 1 of the PGK1 gene were mapped by ligation-mediated polymerase chain reaction. Repair rates within the nontranscribed strand varied as much as 15-fold, depending on nucleotide position. Preferential repair of the transcribed strand began just downstream of the transcription start site but was most pronounced beginning at nucleotide +140 in exon 1. The promoter contained two slowly repaired regions that coincided with two transcription factor binding sites.