Rapid Prototyping a Double-layer Polyurethane-collagen Conduit and its Schwann Cell Compatibility
Rapid Prototyping a Double-layer Polyurethane-collagen Conduit and its Schwann Cell Compatibility
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DOI：
10.1177/0883911509102349
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发表时间：
2009-05-01
影响因子：
1.7
通讯作者：
Zhang, Renji
中科院分区：
文献类型：
--
作者：
Cui, Tongkui;Wang, Xiaohong;Zhang, Renji
采用双喷嘴低温沉积制造（DLDM）技术，制备了一种外层合成聚氨酯（PU）层和内层天然胶原蛋白层相结合的新型双层导管。体外PU层为体内抵抗瘢痕组织的侵袭提供了一个机械稳定的通道，而内层胶原蛋白层促进了雪旺细胞的粘附、迁移和增殖。在两层中均发现微孔结构。通过调整两个沉积喷嘴之间的距离和调整其他工艺参数，实现了双层之间的紧密连接。大鼠坐骨神经雪旺细胞在pu -胶原层状导管中培养1周；与纯PU相比，其保留率和活力显著提高。聚氨酯胶原双层导管具有良好的雪旺细胞相容性，在临床周围神经修复中有很大的应用潜力。
A new double-layer conduit that combined an outer synthetic polyurethane (PU) layer and inner natural collagen layer was fabricated via a double-nozzle low-temperature deposition manufacturing (DLDM) technology. The outer PU layer provided a mechanically stable tunnel against scar tissue invasion in vivo, while the inner collagen layer promoted Schwann cell adhesion, migration, and proliferation. Microporous structures were found in both the layers. A tight connection between the double layers was achieved by adjusting the distance between the two deposit nozzles and adjusting other processing parameters. Schwann cells from rat sciatic nerves were cultured in the layered PU-collagen conduits for one week; a significant enhancement in their retention and viability was seen compared to those made of pure PU. The poly(urethane collagen) double layer conduit had better Schwann cell compatibility and so has a great potential use in clinical peripheral nerve repair.