Gal-Gal pili vaccines prevent pyelonephritis by piliated Escherichia coli in a murine model. Single-component Gal-Gal pili vaccines prevent pyelonephritis by homologous and heterologous piliated E. coli strains.
Gal-Gal pili vaccines prevent pyelonephritis by piliated Escherichia coli in a murine model. Single-component Gal-Gal pili vaccines prevent pyelonephritis by homologous and heterologous piliated E. coli strains.
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Gal-Gal 菌毛疫苗可在小鼠模型中预防由毛状大肠杆菌引起的肾盂肾炎。
DOI：
10.1172/jci114123
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
O'Hanley,P
中科院分区：
文献类型：
--
作者：
Pecha,B;Low,D;O'Hanley,P
非梗阻性大肠杆菌肾盂肾炎的初始致病步骤通常涉及细菌粘附素与宿主尿上皮糖蛋白受体的结合，该受体含有D-Gal p α 1----4 D-Gal p β 1 （Gal-Gal）片段。在这项研究中，用Gal-Gal菌毛免疫各组小鼠，并在2周后用表达同源或异源菌毛的大肠杆菌菌株进行囊内攻击。129只经毛免疫的小鼠中有63只（49%）受到保护，不受随后大肠杆菌在肾脏定植的影响，而85只对照小鼠中有5只（6%）受到保护。在右肾培养大肠杆菌的小鼠中，对照组小鼠的细菌集落计数高于毛免疫小鼠（P < 0.05）。光镜检查显示毛毛免疫小鼠肾脏组织病理学改变少于对照组（P < 0.05）。这种保护作用与尿和血清中特异性IgG抗体的存在有关，这些抗体与主要的毛蛋白结构多肽结合，而不是与Gal-Gal毛尖粘附素本身结合。这些结果支持了细菌表面涂层成分免疫可以通过干扰定植来预防粘膜感染的概念。此外，大肠杆菌的Gal-Gal菌毛是预防肾盂肾炎的合适候选物。
The initial pathogenic step in nonobstructive Escherichia coli pyelonephritis usually involves the binding of a bacterial adhesin with host uroepithelial glycoprotein receptors containing the D-Gal p alpha 1----4 D-Gal p beta 1 (Gal-Gal) moiety. In this study, groups of mice were immunized with Gal-Gal pili and challenged 2 wk later intravesicularly with E. coli strains expressing homologous or heterologous pili. 63 of 129 pili-immunized mice (49%) were protected from subsequent E. coli renal colonization compared with 5 of 85 control mice (6%). Among mice that had E. coli cultured from their right kidney, control mice had greater bacterial colony counts than pili-immunized animals (P less than 0.05). Light microscopic examination of kidneys demonstrated less histopathology among pili immunized mice than among control mice (P less than 0.05). Protection correlated with the presence of specific IgG antibodies in the urine and serum that bind to the major pilin structural polypeptide and not to the Gal-Gal pili tip adhesin per se. These results support the concept that immunization with a bacterial surface-coat constituent can prevent mucosal infection by interfering with colonization. Also Gal-Gal pili of E. coli represent a suitable candidate for immunoprophylaxis against pyelonephritis.