Deletion of the gene encoding the glycolytic enzyme triosephosphate isomerase (tpi) alters morphology of Salmonella enterica serovar Typhimurium and decreases fitness in mice.
Deletion of the gene encoding the glycolytic enzyme triosephosphate isomerase (tpi) alters morphology of Salmonella enterica serovar Typhimurium and decreases fitness in mice.
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删除编码糖酵解酶磷酸三糖异构酶（tpi）的基因会改变鼠伤寒沙门氏菌的形态并降低小鼠的健康水平。
DOI：
10.1111/j.1574-6968.2009.01553.x
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发表时间：
2009
影响因子：
2.1
通讯作者：
Paterson GK
中科院分区：
文献类型：
--
作者：
Paterson GK
The glycolytic enzyme triosephosphate isomerase (tpi) (EC 5.3.1.1) plays a key role in central carbon metabolism yet few studies have characterized isogenic bacterial mutants lacking this enzyme and none have examined its role in thein vivofitness of a bacterial pathogen. Here we have deletedtpiAinSalmonella entericaserovar Typhimurium and found that the mutant had an altered morphology, displaying an elongated shape compared with the wild type. In a mouse model of typhoid fever thetpiAmutant was attenuated for growth as assessed by bacterial counts in the livers and spleens of infected mice. However, this attenuation was not deemed sufficient for consideration of atpiAmutant as a live attenuated vaccine strain. These phenotypes were complemented by provision oftpiAon pBR322. We therefore provide the first demonstration thattpiAis required for fullin vivofitness of a bacterial pathogen, and that it has a discernable impact on cell morphology.
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