Sexually dimorphic neurocalcin expression in the developing zebra finch telencephalon.
Sexually dimorphic neurocalcin expression in the developing zebra finch telencephalon.
复制标题
DOI：
10.1002/neu.10246
复制
发表时间：
2003-09
期刊：
影响因子：
--
通讯作者：
S. Veney;Camilla Peabody;George W. Smith;J. Wade
中科院分区：
文献类型：
--
作者：
S. Veney;Camilla Peabody;George W. Smith;J. Wade
从斑雀端脑中提取的RNA进行了差异显示RT-PCR，以确定可能与其发育有关的基因产物，包括负责歌曲学习和产生的性二形核。克隆并测序了一个仅在幼年雌性中发现的cdna。它与钙结合蛋白神经钙素有同源性。Northern杂交显示有三种神经钙素。一个10.6kb的转录本存在于男性和大多数女性的整个发育和成年期。较小的6.2和3.3kb物种几乎完全在雌性中检测到，主要是在孵化后18-25天之间。原位杂交表明，这三种基因在两性的端脑中都有广泛的分布，特别是在宋氏核的RA中表达水平较高。在研究的各个区域中，与男性相比，女性神经钙素的表达增强，这可能反映了两个较小转录本的存在。然而，在RA中，神经钙素在性别之间的表达在统计学上是相同的。这些数据表明，通过神经钙素的钙信号可能参与了端脑的发育，但表明该基因的性别二态表达太普遍了，无法专门调节歌曲控制区的男性或女性发育。Neurocalin可能：广泛影响功能分化，包括性别之间在形态上不同的区域；是一般二态的良性结果，例如由性染色体引起的；或涉及代偿机制，允许幼年女性端脑的功能与男性相同，尽管存在根本的生理差异。
Differential display RT-PCR was used on RNA isolated from the zebra finch telencephalon to identify gene products potentially involved in its development, including the sexually dimorphic nuclei responsible for song learning and production. A cDNA identified only in juvenile females was cloned and sequenced. It shares homology with neurocalcin, a calcium binding protein. Northern blots indicated three neurocalcin species. A 10.6 kb transcript was present in males and most females throughout development and in adulthood. Smaller 6.2 and 3.3 kb species were detected almost exclusively in females and primarily between posthatching days 18-25. In situ hybridization, using a probe that identified all three mRNA species, indicated a broad distribution in the telencephalon of both sexes, with particularly high levels in the song nucleus RA. Across regions examined, neurocalcin expression was enhanced in females compared to males, probably reflecting the presence of the two smaller transcripts. However, within RA, neurocalcin expression was statistically equivalent between the sexes. These data indicate that calcium signaling via neurocalcin may be involved in telencephalic development, but suggest that sexually dimorphic expression of this gene exists on a level too general to specifically regulate masculine or feminine development of song control regions. Neurocalcin might: broadly influence functional differentiation, including areas that are not morphologically distinct between the sexes; be a benign consequence of general dimorphisms, such as those due to sex chromosomes; or involve a compensatory mechanism, which allows function of the juvenile female telencephalon to equal that of males, despite fundamental physiological differences.