Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper.
Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper.
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DOI：
10.1039/c1lc20758j
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发表时间：
2011-12-21
期刊：
影响因子：
6.1
通讯作者：
Yager P
中科院分区：
文献类型：
--
作者：
Lutz BR;Trinh P;Ball C;Fu E;Yager P
大多数实验室分析利用多步骤方案来实现高性能，但传统的纸基测试(例如，侧向流动测试)通常仅限于可在单个流体步骤中执行的分析。我们已经开发了二维纸质网络(2DPN)，它使用来自横向流动测试的材料，但重新配置它们，以便能够对多步骤试剂输送序列进行编程。2DPN使用多个汇聚的流体入口来控制每个流体到达检测区或反应区的时间，并且它需要一种方法来以相应的定时顺序断开每个流体源。在这里，我们提出了一种方法，允许编程断开多步骤输送所需的流体源。将具有不同长度的腿的2DPN插入到共享缓冲井中，并且滴液表面以可编程的顺序断开每个腿At。这种方法可以使多步骤实验室分析转换为简单的护理点设备，具有高性能，但仍然易于使用。
Most laboratory assays take advantage of multi-step protocols to achieve high performance, but conventional paper-based tests (e.g., lateral flow tests) are generally limited to assays that can be carried out in a single fluidic step. We have developed two-dimensional paper networks (2DPNs) that use materials from lateral flow tests but reconfigure them to enable programming of multi-step reagent delivery sequences. The 2DPN uses multiple converging fluid inlets to control the arrival time of each fluid to a detection zone or reaction zone, and it requires a method to disconnect each fluid source in a corresponding timed sequence. Here, we present a method that allows programmed disconnection of fluid sources required for multi-step delivery. A 2DPN with legs of different lengths is inserted into a shared buffer well, and the dropping fluid surface disconnects each leg at in a programmable sequence. This approach could enable multi-step laboratory assays to be converted into simple point-of-care devices that have high performance yet remain easy to use.