Sulfate and phosphate analysis in glycoproteins and other biologic compounds using ion chromatography. Application to glycoprotein hormones and sugar esters.
Sulfate and phosphate analysis in glycoproteins and other biologic compounds using ion chromatography. Application to glycoprotein hormones and sugar esters.
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使用离子色谱法分析糖蛋白和其他生物化合物中的硫酸盐和磷酸盐。
DOI：
10.1016/s0021-9673(01)96511-2
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Bousfield,GR
中科院分区：
文献类型：
--
作者：
Ward,DN;Wen,T;Bousfield,GR
设计了离子色谱程序用于同时测定同一样品中的磷酸盐和硫酸盐。为了消除含磷酸盐或硫酸盐化合物水解过程中产生的两性离子化合物（特别是氨基酸和肽）的干扰，需要使用阳离子交换柱进行预处理步骤。以摩尔计算，硫酸盐的检测灵敏度大约是磷酸盐的两倍。使用离子色谱法时，硫酸盐的有用工作范围为 200 pmole 至 35 nmole；磷酸盐的范围是 400 pmole 到 65 nmole。在此范围内的线性度非常令人满意。对几种糖蛋白激素以及糖硫酸盐和磷酸酯的水解产物进行了代表性分析。重复分析为±3.0% 或更好。硫酸盐的糖蛋白激素分析并未表明每摩尔的整数，这表明正如其他人使用色谱聚焦或等电聚焦和免疫测定法所发现的那样，是异激素的混合物。
An ion chromatography procedure was devised for the simultaneous determination of phosphate and sulfate in the same sample. In order to eliminate interference from zwitterionic compounds (particularly amino acids and peptides) generated during hydrolysis of the phosphate- or sulfate-containing compounds a pretreatment step with a cation-exchange column was required. The detection of sulfate is approximately twice as sensitive as phosphate on a molar basis. The useful working range for sulfate was 200 pmole to 35 nmole with the ion chromatography employed; the range for phosphate was 400 pmole to 65 nmole. Linearity in this range was very satisfactory. Representative analyses are presented for hydrolyzates of several glycoprotein hormones and sugar sulfates and phosphate esters. Replicate analyses were ±3.0% or better. The glycoprotein hormone analyses for sulfate did not indicate whole integers per mole, suggesting mixtures of isohormones as has been found by others using chromatofocusing or isoelectric focussing and immunoassay.