Reactive morphology of dividing microglia following kainic acid administration.
Reactive morphology of dividing microglia following kainic acid administration.
复制标题
DOI：
10.3389/fnins.2022.972138
复制
发表时间：
2022
影响因子：
4.3
通讯作者：
Rowe, Rachel K.
中科院分区：
文献类型：
--
作者：
Green, Tabitha R. F.;Murphy, Sean M.;Moreno-Montano, Maria P.;Audinat, Etienne;Rowe, Rachel K.
小胶质细胞对病理微环境的反应以细胞形态学变化为标志。在病理刺激后，小胶质细胞变得反应性，同时分裂产生子细胞。虽然已经观察到广泛的小胶质细胞形态，这些不同的形态的确切功能是未知的，因为是分裂小胶质细胞的形态和反应状态。在这项研究中，我们使用红藻氨酸触发小胶质细胞激活和细胞分裂。在皮质红藻氨酸注射后，在注射后3天，使用离子钙结合衔接分子1（Iba1）的免疫组织化学对小胶质细胞进行染色，并使用KI 67作为细胞分裂的标志物，检查小胶质细胞的形态和增殖。从显微照片中分离出单个小胶质细胞，并使用骨架和分形分析来检查细胞大小和空间复杂性。我们研究了野生型和小胶质细胞特异性肿瘤坏死因子（TNF）-α敲除小鼠中小胶质细胞的形态学。使用广义线性混合模型或双因素ANOVA分析数据。我们发现，与未分裂的小胶质细胞相比，分裂的小胶质细胞具有更多的反应性形态（更大的细胞体面积，更长的细胞周长和更少的分支），无论小胶质细胞是否释放TNF-α。然而，我们也观察到分裂的小胶质细胞具有复杂的，更分枝的形态。小胶质细胞形态和分裂的变化最大的红藻氨酸注射部位附近。这项研究使用强大的定量技术来更好地了解小胶质细胞分裂，形态和群体动态，这对于开发针对小胶质细胞的新型疗法至关重要。
The microglial response to a pathological microenvironment is hallmarked by a change in cellular morphology. Following a pathological stimulus, microglia become reactive and simultaneously divide to create daughter cells. Although a wide array of microglial morphologies has been observed, the exact functions of these distinct morphologies are unknown, as are the morphology and reactivity status of dividing microglia. In this study, we used kainic acid to trigger microglial activation and cell division. Following a cortical kainic acid injection, microglial morphology and proliferation were examined at 3 days post-injection using immunohistochemistry for ionized calcium binding adapter molecule 1 (Iba1) to stain for microglia, and KI67 as a marker of cell division. Individual microglial cells were isolated from photomicrographs and skeletal and fractal analyses were used to examine cell size and spatial complexity. We examined the morphology of microglia in both wildtype and microglia-specific tumor necrosis factor (TNF)-α knockout mice. Data were analyzed using generalized linear mixed models or a two-way ANOVA. We found that dividing microglia had a more reactive morphology (larger cell body area, longer cell perimeter, and less ramification) compared to microglia that were not dividing, regardless of microglial release of TNF-α. However, we also observed dividing microglia with a complex, more ramified morphology. Changes in microglial morphology and division were greatest near the kainic acid injection site. This study uses robust and quantitative techniques to better understand microglial cell division, morphology, and population dynamics, which are essential for the development of novel therapeutics that target microglia.
登录
查看更多内容
耦合的增殖和凋亡维持成人大脑中小胶质细胞的快速离职。
影响因子：
8.8
作者：
Askew K;Li K;Olmos-Alonso A;Garcia-Moreno F;Liang Y;Richardson P;Tipton T;Chapman MA;Riecken K;Beccari S;Sierra A;Molnár Z;Cragg MS;Garaschuk O;Perry VH;Gomez-Nicola D
通讯作者：
Gomez-Nicola D
影响因子：
5.3
作者：
Avignone, Elena;Ulmann, Lauriane;Audinat, Etienne
通讯作者：
Audinat, Etienne
影响因子：
2.7
作者：
Cao Y;Wang Y;Li C;Jiang Q;Zhu L
通讯作者：
Zhu L
影响因子：
2.5
作者：
Akahoshi, Noriyuki;Murashima, Yoshiya L.;Ishii, Isao
通讯作者：
Ishii, Isao
影响因子：
6.1
作者：
Abraham, Jayne;Fox, Patrick D.;Condello, Carlo;Bartolini, Alyssa;Koh, Sookyong
通讯作者：
Koh, Sookyong