Development and evaluation of a novel decellularized vascular xenograft
Development and evaluation of a novel decellularized vascular xenograft
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DOI：
10.1016/s1350-4533(02)00010-3
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发表时间：
2002-04-01
影响因子：
2.2
通讯作者：
Chen, C
中科院分区：
文献类型：
--
作者：
Conklin, BS;Richter, ER;Chen, C
尽管自体隐静脉仍然是冠状动脉和腘窝下搭桥术的标准，但许多患者没有合适的静脉。开发小口径血管移植物的尝试主要由于闭塞、新内膜增生或动脉瘤退化而失败。我们设计并表征了一种新型小口径血管异种移植物，可以克服这些失败模式。为了减少免疫反应，通过酶和去污剂处理对猪颈总动脉进行脱细胞处理。组织学和电子显微镜检查显示细胞成分完全去除，而细胞外基质结构保持完整。为了减少血栓形成，脱细胞血管移植物与肝素共价连接。通过甲苯胺蓝染色证明了肝素连接的效率，并通过凝血时间测试证明了肝素处理的移植物的抗血栓形成性。对移植物进行机械测试。脱细胞肝素处理的移植物与新鲜血管的顺应性相似，爆裂测试显示移植物能够承受超过生理血压 10 倍的压力。新鲜血管和脱细胞肝素处理的移植物之间的缝合保留强度没有差异。脱细胞、肝素化的移植物被植入狗体内作为颈动脉旁路移植物，两个月后显示平滑肌细胞密集地分布在血管壁上，内皮细胞排列在管腔内。这项研究为开发具有优异机械性能、抗血栓形成性和组织相容性的小口径血管移植物提供了新策略。 (C) 2002 年 IPEM。由爱思唯尔科学有限公司出版。保留所有权利。
Although autogenous saphenous vein remains the standard for coronary and infrapopliteal bypass, many patients do not have a suitable vein. Attempts at developing a small-caliber vascular graft have failed largely due to occlusion, neointimal hyperplasia, or aneurismal degradation. We have designed and characterized a novel small-caliber vascular xenograft that may overcome these failure modes. To reduce immune reactions, porcine common carotid arteries were decellularized by enzymatic and detergent treatments. Histology and electron microscopic examination showed complete removal of cellular components while the extracellular matrix structure remained intact. To reduce thrombogeneity, decellularized vascular grafts were covalently linked with heparin. The efficiency of heparin linkage was demonstrated with toluidine blue staining and the antithrombogeneity of the heparin-treated grafts was demonstrated with a clot time test. Mechanical testing of the graft was performed. Decellularized-heparin-treated grafts were similar in compliance to fresh vessels and burst testing showed grafts to withstand pressures exceeding 10 times physiologic blood pressure. There was no difference in suture retention strength between fresh vessels and decellularized-heparin-treated grafts. Decellularized, heparinized grafts were implanted in dogs as carotid artery bypass grafts and showed smooth muscle cells densely populating the wall, and endothelial cells lining the lumen by two months. This study provides a new strategy to develop a small-caliber vascular graft with excellent mechanical properties, antithrombogeneity, and tissue compatibility. (C) 2002 IPEM. Published by Elsevier Science Ltd. All rights reserved.