Effect of eccentric contraction on satellite cell activation in human vastus lateralis muscle
Effect of eccentric contraction on satellite cell activation in human vastus lateralis muscle
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DOI：
10.1007/s12576-015-0385-4
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发表时间：
2015-09-01
影响因子：
2.3
通讯作者：
Miyata, Hirofumi
中科院分区：
文献类型：
--
作者：
Imaoka, Yoko;Kawai, Minako;Miyata, Hirofumi
我们比较了步进运动后股外侧肌（VL）离心和同心收缩的卫星细胞（SC）激活的时间过程。年轻人参加了一个30分钟的步升/降运动，主要涉及与右VL肌肉和离心收缩与左VL肌肉的同心收缩。通过肌电图（EMG）和膝关节角度的变化来确定VL肌肉的同心和离心收缩相。在三个时间段从两个VL肌肉采集活检样品：运动前和运动后2天和5天。我们发现，SC的数量显着增加，在IIa型纤维的左VL在运动后2和5天。肝细胞生长因子（HGF）和肌源性分化因子1（MyoD）mRNA的表达在运动后第2天和第5天在左侧VL中显著增加，在运动后第5天在右侧VL中显著增加。运动后第2天，左侧VL瞬时受体电位典型（TRPC）1 mRNA的表达也增加。这些结果表明，离心收缩可以有效地激活SC增殖长达5天后的运动。HGF、MyoD和TRPC 1 mRNA表达的类似变化表明，通过阳离子流入的HGF/c-Met信号激活对响应离心运动的骨骼肌SC激活具有重大影响。
We compared the time-course of satellite cell (SC) activation between eccentric and concentric contractions in the vastus lateralis (VL) muscle after step exercise. Young adults participated in a 30-min step up/down exercise which mainly involved concentric contractions with the right VL muscle and eccentric contractions with the left VL muscle. The concentric and eccentric contraction phases of the VL muscles were identified by changes in the electromyogram (EMG) and knee joint angle. Biopsy samples were taken from both VL muscles at three time periods: before the exercise and 2 and 5 days after the exercise. We found that the numbers of SCs were significantly increased in the type IIa fibers of the left VL at 2 and 5 days after the exercise. The expression of both hepatocyte growth factor (HGF) and myogenic differentiation 1 (MyoD) mRNA had significantly increased in the left VL at 2 and 5 days after the exercise and in the right VL at 5 days after the exercise. The expression of transient receptor potential canonical (TRPC) 1 mRNA also increased in the left VL at 2 days after exercise. These results indicate that eccentric contraction can effectively activate SC proliferation for up to 5 days after exercise. Similar changes in HGF, MyoD and TRPC1 mRNA expression suggest that HGF/c-Met signal activation through cation influx has a major impact on skeletal muscle SC activation in response to eccentric exercise.