Stochastic and genetic factors influence tissue-specific decline in ageing C-elegans
Stochastic and genetic factors influence tissue-specific decline in ageing C-elegans
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DOI：
10.1038/nature01135
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发表时间：
2002-10-24
期刊：
影响因子：
64.8
通讯作者：
Driscoll, M
中科院分区：
文献类型：
--
作者：
Herndon, LA;Schmeissner, PJ;Driscoll, M
秀丽隐杆线虫是研究衰老遗传学的重要模型，迄今已知有50多种延长寿命的突变。然而，很少有人知道在这个物种的衰老的病理生物学，限制尝试连接基因型与衰老表型。使用超微结构分析和特定细胞类型的可视化与绿色荧光蛋白，我们检查了不同组织中的细胞完整性，随着动物的年龄。我们报告显着的神经系统的保护，即使在先进的老年人，与肌肉的逐渐，进行性恶化，类似于人类的肌肉减少症。age-1（hx 546）突变可以延长60- 100%的寿命，延迟了一些（但不是全部）衰老的细胞生物标志物。引人注目的是，我们发现了强有力的证据表明，随机以及遗传因素是显着的C。线虫衰老的过程中，在相同年龄的动物之间和个体内相同类型的细胞之间都存在广泛的变异性。
The nematode Caenorhabditis elegans is an important model for studying the genetics of ageing, with over 50 life-extension mutations known so far. However, little is known about the pathobiology of ageing in this species, limiting attempts to connect genotype with senescent phenotype. Using ultrastructural analysis and visualization of specific cell types with green fluorescent protein, we examined cell integrity in different tissues as the animal ages. We report remarkable preservation of the nervous system, even in advanced old age, in contrast to a gradual, progressive deterioration of muscle, resembling human sarcopenia. The age-1 (hx546) mutation, which extends lifespan by 60-100%, delayed some, but not all, cellular biomarkers of ageing. Strikingly, we found strong evidence that stochastic as well as genetic factors are significant in C. elegans ageing, with extensive variability both among same-age animals and between cells of the same type within individuals.