Effects of temperature increase on the grazing of planktonic bacteria by biofilm-dwelling consumers
Effects of temperature increase on the grazing of planktonic bacteria by biofilm-dwelling consumers
复制标题
DOI：
10.3354/ame01286
复制
发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Weitere, Markus
中科院分区：
文献类型：
--
作者：
Kathol, Marcel;Norf, Helge;Weitere, Markus
生物膜是一种复杂的微生物群落，它与水体表面紧密相连，在沃茨的物质流动中起着重要的作用。除了细菌和藻类，异养原生生物和小型后生动物也可以在生物膜中以高丰度出现。通过吃水生猎物，生物膜栖息的食草动物可以作为水生和底栖食物网之间的营养链，与滤食性底栖大型无脊椎动物平行。在这里，我们介绍了一种流动池的方法，半天然生物膜悬浮细菌的放牧率的量化，平行于微观生物膜群落分析。这种技术被用来测量复杂的生物膜群落（建立在旁路系统的莱茵河，德国）的放牧率对嗜热菌的温度响应。结果表明，在室温下，14 d龄的生物膜（主要为纤毛虫）清除浮游细菌的速率在29（冬季）和99 1 m（-2）生物膜d（-1）（夏季）之间，这些速率随温度的升高而增加，即使在温和气候下滤食性大型底栖动物的摄食速率受到负面影响的温度（> 30 ℃）下也是如此。驯化温度下，生物膜的发展可以有一个显着的影响，社区清除率，主要是由于温度的影响，丰富和结构的食草动物群落，而没有迹象表明驯化影响个人放牧率可以找到。生活在生物膜中的消费者群体对温度变化的反应比大型底栖滤食性动物快得多。
Biofilms, the complex microbial communities associated with surfaces, can play an important role in the flux of matter in running waters. In addition to bacteria and algae, heterotrophic protists and small metazoans can also occur in high abundances in biofilms. By grazing on planktonic prey, biofilm-dwelling grazers can act as a trophic link between planktonic and benthic food webs, parallel to filter-feeding benthic macroinvertebrates. Here we introduce a flow cell method for the quantification of grazing rates of semi-natural biofilms on suspended bacteria, parallel to microscopic biofilm community analysis. This technique was used to measure the temperature response of the grazing rates of complex biofilm communities (established in a bypass system to the River Rhine, Germany) on planktonic bacteria. Results showed that 14 d old biofilms (dominated by ciliates) clear planktonic bacteria at rates between 29 (in winter) and 99 1 m(-2) biofilm d(-1) (in summer) at ambient temperature, These rates increased with test temperature, even at temperatures (> 30 degrees C) at which the grazing rates of filter-feeding macrofauna in moderate climates are negatively influenced. The acclimation temperatures under which the biofilm developed can have a significant effect on community clearance rates, largely due to a temperature effect on the abundance and structure of the grazer community, whereas no indication of an acclimation effect on individual grazing rates could be found. In the function of bentho-pelagic couplers, biofilm-dwelling consumer communities can react to temperature changes substantially faster than macrofaunal filter feeders.