A simple method for quantifying Leishmania in tissues of infected animals
A simple method for quantifying Leishmania in tissues of infected animals
复制标题
DOI：
10.1016/s0169-4758(96)40010-2
复制
发表时间：
1997-02-01
期刊：
影响因子：
--
通讯作者：
Titus, RG
中科院分区：
文献类型：
--
作者：
Lima, HC;Bleyenberg, JA;Titus, RG
在感染主要利什曼原虫的实验动物中，寄生虫皮肤病变的大小通常与病变内寄生虫的数量无关。事实上，皮肤损伤可以愈合，但仍然含有寄生虫。因此，确定受感染动物的寄生虫负担的能力变得重要，特别是在评估旨在诱导灭菌免疫的疫苗时。在这里，赫梅尼奥·利马、朱莉·布莱恩伯格和理查德·蒂图斯描述了一种在感染组织中计数利什曼原虫的简单技术。人们希望这项技术将使所有研究利什曼病的研究人员(特别是那些利什曼病流行国家的研究人员)能够轻松地确定受感染动物的寄生虫负担。
In experimental animals infected with Leishmania major, the size of cutaneous lesions of the parasite often does not correlate with the number of parasites within the lesion. Indeed, cutaneous lesions can heal, but still contain parasites. Thus, the ability to determine parasite burden in infected animals becomes important, especially when assessing vaccines that are intended to induce sterilizing immunity. Here, Hermenio Lima, Julie Bleyenberg and Richard Titus describe a simple technique for enumerating Leishmania in infected tissue. It is hoped that this technique will allow all researchers working with Leishmania (especially those in countries where leishmaniasis is endemic) to determine parasite burden easily in infected animals.