PROTECTIVE IMMUNITY TO ROTAVIRUS-INDUCED DIARRHEA IS PASSIVELY TRANSFERRED TO NEWBORN MICE FROM NAIVE DAMS VACCINATED WITH A SINGLE DOSE OF A RECOMBINANT ADENOVIRUS EXPRESSING ROTAVIRUS VP7SC
PROTECTIVE IMMUNITY TO ROTAVIRUS-INDUCED DIARRHEA IS PASSIVELY TRANSFERRED TO NEWBORN MICE FROM NAIVE DAMS VACCINATED WITH A SINGLE DOSE OF A RECOMBINANT ADENOVIRUS EXPRESSING ROTAVIRUS VP7SC
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DOI：
10.1006/viro.1993.1203
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发表时间：
1993-04-01
期刊：
影响因子：
3.7
通讯作者：
ANDREW, ME
中科院分区：
文献类型：
--
作者：
BOTH, GW;LOCKETT, LJ;ANDREW, ME
VP7sc 是一种新型轮状病毒抗原，设计用于在细胞表面呈递。构建了几种重组病毒，其中将 VP7sc 插入人 5 型腺病毒 (Ad5) 基因组的 E3 区域，并在培养的 293 细胞中监测抗原的表达和转运。然后使用表现出最大表达水平(Ad5/7.4)的重组病毒来确定是否可以在非人类宿主中诱导针对VP7sc的抗体。通过静脉注射、腹腔注射、口服和鼻内途径给BALB/c和CBA/H小鼠接种Ad5/7.4，并通过ELISA测定血清抗体水平。所有接种疫苗的动物均出现血清转化，但根据疫苗接种途径，并非所有动物在重新接种后都表现出显着的继发反应。还使用多种疫苗接种方案检查了 Ad5/7.4 在小鼠中诱导保护性免疫的能力。向先前未接触过轮状病毒的母鼠鼻内给予单剂 Ad5/7.4 足以诱导免疫力，这种免疫力可以被动转移以保护哺乳新生儿。因此，表达保护性抗原的重组腺病毒可以为开发抗胃肠炎疫苗提供使用减毒轮状病毒的替代方案。
VP7sc is a novel rotavirus antigen engineered for presentation at the cell surface. Several recombinant viruses were constructed in which VP7sc was inserted into the E3 region of the human type 5 adenovirus (Ad5) genome and expression and transport of the antigen was monitored in cultured 293 cells. The recombinant virus showing the greatest level of expression (Ad5/7.4) was then used to determine whether antibodies to VP7sc could be induced in a nonhuman host. BALB/c and CBA/H mice were inoculated with Ad5/7.4 by iv, ip, oral and intranasal routes and serum antibody levels were assayed by ELISA. All vaccinated animals seroconverted but, depending on the route of vaccination, not all animals showed a significant secondary response following re-inoculation. The ability of Ad5/7.4 to induce protective immunity in mice was also examined using several vaccination regimes. A single dose of Ad5/7.4 given intranasally to dams not previously exposed to rotavirus was sufficient to induce immunity which could be passively transferred to protect suckling neonates. Recombinant adenoviruses expressing protective antigens therefore may provide an alternative to the use of attenuated rotaviruses in the development of a vaccine against gastroenteritis.