Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons.
Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons.
复制标题
用于监测多巴胺能神经元翻译的小鼠系的生成和表征。
DOI：
10.1038/s41598-017-08618-2
复制
发表时间：
2017
影响因子：
4.6
通讯作者：
Dougherty,JosephD
中科院分区：
文献类型：
--
作者：
Dougherty,JosephD
We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain dopamine neurons and validation of its effectiveness in capturing mRNA from these cells. We also report a translational profile of these neurons which may be of use to investigators studying the gene expression of these cells. Finally, we have provided the line to Jackson Laboratories for distribution and use in future studies.
