Parotid acinar cells transiently change to duct-like cells during epithelial-mesenchymal transition
Parotid acinar cells transiently change to duct-like cells during epithelial-mesenchymal transition
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DOI：
10.2152/jmi.56.258
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发表时间：
2009-12-01
影响因子：
0.7
通讯作者：
Sugiya, Hiroshi
中科院分区：
文献类型：
--
作者：
Fujita-Yoshigaki, Junko;Qi, Bing;Sugiya, Hiroshi
唾液分泌减少和随之而来的口干会导致严重的龋齿和牙周病。头颈癌和涎腺炎的放射治疗可导致唾液腺萎缩和纤维化，但其机制尚不清楚。作为唾液腺功能障碍的模型，我们检测了原代培养的腮腺腺泡细胞基因表达谱的变化。淀粉酶和水通道蛋白-5等腺泡标志物的表达水平在培养过程中迅速下降。与此同时，导管标记物开始表达，尽管其表达是短暂的。在培养后期，上皮-间质转化标志物开始表达和增加。Src或p38MAP激酶抑制剂抑制了这些变化。这些结果表明腮腺腺泡细胞在上皮间质转化过程中会短暂地转变为导管样细胞，这种转变是通过Src-p38 MAP激酶通路的信号转导诱导的。腮腺腺泡细胞有可能保持分化的可塑性。
Hyposecretion of saliva and consequent dry mouth lead to severe caries and periodontal disease. Therapeutic radiation for head and neck cancer and sialadenitis result in atrophy and fibrosis of salivary glands, but the mechanism is not clear. As a model for dysfunction of salivary glands, we examined the change of gene expression patterns in primary cultured parotid acinar cells. The expression levels of acinar markers such as amylase and aquaporin-5 rapidly decreased during culture. At the same time, ductal markers began to be expressed although their expression was transient. In the late phase of culture, markers of epithelial-mesenchymal transition began to be expressed and increased. Inhibitor for Src or p38MAP kinase suppressed these changes. These results suggest that parotid acinar cells transiently change to duct-like cells during epithelialmesenchymal transition and that these changes are induced by signal transduction via Src-p38 MAP kinase pathway. There is a possibility that parotid acinar cells retain a plasticity of differentiation.