Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital
Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital
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DOI：
10.1093/jac/dkg140
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发表时间：
2003-03-01
影响因子：
5.2
通讯作者：
Kim, S
中科院分区：
文献类型：
--
作者：
Yun, HJ;Lee, SW;Kim, S
莫匹罗星已用于对抗革兰氏阳性病原菌，是细菌异亮氨酰-tRNA 合成酶的特异性抑制剂。在这项工作中，我们确定了从一家韩国医院分离出来的葡萄球菌中莫匹罗星耐药性的流行情况，并调查了耐药性的特征。在金黄色葡萄球菌中，高水平莫匹罗星耐药率为 5%（319 例中有 16 例），而未检测到低水平莫匹罗星耐药。在凝固酶阴性葡萄球菌 (CoNS) 中，高水平和低水平莫匹罗星耐药率分别为 16.7%（204 例中的 34 例）和 10.3%（204 例中的 21 例）。高水平耐药菌株含有ileS-2基因，该基因编码一种新型葡萄球菌异亮氨酰-tRNA合成酶。相比之下，所有低水平莫匹罗星耐药CoNS都含有突变V588F，该突变位于染色体葡萄球菌异亮氨酰-tRNA合成酶基因（ileS）内的保守基序KMSKS附近。总之，这项工作描述了韩国最近但迅速出现的两种不同类型的莫匹罗星耐药葡萄球菌，以及 CoNS 异亮氨酰-tRNA 合成酶的序列和突变体特征。
Mupirocin has been used against Gram-positive pathogenic bacteria, and is a specific inhibitor of bacterial isoleucyl-tRNA synthetase. In this work, we have determined the prevalence of mupirocin resistance among staphylococci isolated from a Korean hospital, and have investigated the characteristics of the resistance. In Staphylococcus aureus, the prevalence of high-level mupirocin resistance was 5% (16 of 319), whereas low-level mupirocin resistance was not detected. In coagulase-negative staphylococci (CoNS) the rates of high- and low-level mupirocin resistance were 16.7% (34 of 204) and 10.3% (21 of 204), respectively. The high-level resistant strains contained the ileS-2 gene, which encodes a novel staphylococcal isoleucyl-tRNA synthetase. In contrast, all of the low-level mupirocin-resistant CoNS contained the mutation V588F, which is located near the conserved motif KMSKS, within the chromosomal staphylococcal isoleucyl-tRNA synthetase gene (ileS). In conclusion, this work describes the recent, but rapid, emergence of two different types of mupirocin-resistant staphylococci in Korea, and the sequence and mutant characterization of the isoleucyl-tRNA synthetase of CoNS.