Comprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry.
Comprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry.
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DOI：
10.1016/j.trac.2014.04.017
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发表时间：
2014-10-01
影响因子：
13.1
通讯作者：
Fiehn, Oliver
中科院分区：
文献类型：
--
作者：
Cajka, Tomas;Fiehn, Oliver
关键词：
基于液相色谱-质谱（LC-MS）的脂质组学在过去十年中取得了巨大发展。本文综述了基于LC-MS的脂质组学的最新进展，涵盖了全球脂质组学分析的所有步骤。通过对185篇文献和应用笔记的回顾，我们可以总结出典型的基于LC-MS的脂质组学方法包括：（1）使用氯仿/MeOH或MTBE/MeOH方案进行提取，两者都添加了涵盖每种脂质类别的内标;（2）使用小于2 μm或2.6-2.8 μm的短微孔柱分离脂质（熔融芯）粒度，C18或C8吸附剂，分析时间<30 min;（3）正离子和负离子模式下的电喷雾电离，使用具有MS/MS能力的高分辨率MS进行全光谱采集。（磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰丝氨酸、磷脂酰甘油）其次是鞘磷脂、二酰基甘油和三酰基甘油以及神经酰胺是最常靶向的脂质种类。
Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has been a subject of dramatic developments over the past decade. This review focuses on state of the art in LC-MS-based lipidomics, covering all the steps of global lipidomic profiling. On the basis of review of 185 original papers and application notes, we can conclude that typical LC-MS-based lipidomics methods involve: (1) extraction using chloroform/MeOH or MTBE/MeOH protocols, both with addition of internal standards covering each lipid class; (2) separation of lipids using short microbore columns with sub-2-μm or 2.6–2.8-μm (fused-core) particle size with C18 or C8 sorbent with analysis time <30 min; (3) electrospray ionization in positive- and negative-ion modes with full spectra acquisition using high-resolution MS with capability to MS/MS. Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines, phosphatidylglycerols) followed by sphingomyelins, di- and tri-acylglycerols, and ceramides were the most frequently targeted lipid species.
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