Structure and sequence of the Cu,Zn Sod gene in the Mediterranean fruit fly, Ceratitis capitata: intron insertion/deletion and evolution of the gene.
Structure and sequence of the Cu,Zn Sod gene in the Mediterranean fruit fly, Ceratitis capitata: intron insertion/deletion and evolution of the gene.
复制标题
地中海果蝇 Ceratitis headata 中 Cu、Zn Sod 基因的结构和序列：内含子插入/缺失以及基因的进化。
DOI：
10.1016/1055-7903(92)90037-h
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发表时间：
1992
影响因子：
4.1
通讯作者：
Ayala,FJ
中科院分区：
文献类型：
--
作者：
Kwiatowski,J;Skarecky,D;Ayala,FJ
用果蝇（Drosophilamelanogaster）cDNA探针从地中海实蝇Ceratitiscapitata基因组文库中克隆了Cu，Zn超氧化物歧化酶（SOD）基因的4kb片段。462个碱基的编码序列与果蝇的编码序列一样长。在过去的1亿年中，双翅目和哺乳动物的氨基酸替换速率大致相同，因此排除了哺乳动物中SOD蛋白质的进化比其他生物高得多的假设（提出这一假设是为了解释Sodover地质时期进化速率的明显加速）。在果蝇中只有一个内含子，而在Ceratitis中编码区被两个内含子中断。系统发育比较表明，第二个内含子存在于共同的双翅目祖先，但在果蝇谱系与其他双翅目分支后不久丢失。对不同动物门、植物和真菌中Sod基因外显子/内含子结构的分析表明，在Sod基因的进化过程中，内含子的插入和缺失都有发生。
We have cloned a 4-kb region encompassing theCu,Zn superoxide dismutase (Sod)gene from a genomic library of the Mediterranean fruit fly,Ceratitis capitata, using a cDNA probe fromDrosophila melanogaster. The coding sequence of 462 bases is equally as long as that inDrosophilaspecies. The rate of amino acid replacement over the past 100 million years is approximately the same in the Diptera and in mammals, thus excluding the hypothesis (proposed to account for an apparent acceleration in rate of evolution ofSodover geological time) that the evolution of the SOD protein is much higher in the mammals than in other organisms. The coding region is interrupted by two introns inCeratitis, whereas only one occurs inDrosophila. Phylogenetic comparisons indicate that the second intron was present in the common dipteran ancestor, but was lost shortly after the divergence of theDrosophilalineage from other Diptera. Analysis of the exon/intron structure ofSodin various animal phyla, plants, and fungi indicates that intron insertions as well as deletions have occurred in the evolution of theSodgene.