In vitro effectiveness of different endodontic irrigants on the reduction of Enterococcus faecalis in root canals
In vitro effectiveness of different endodontic irrigants on the reduction of Enterococcus faecalis in root canals
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不同牙髓冲洗剂减少根管内粪肠球菌的体外效果
DOI：
10.4317/jced.2.e169
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发表时间：
2010
影响因子：
--
通讯作者：
S. Himagiri
中科院分区：
文献类型：
--
作者：
S. Vijaykumar;M. Gunashekhar;S. Himagiri
目标：
为了比较蒸馏水灌溉产生的根管内粪肠球菌数量的减少情况，
过氧化氢、次氯酸钠、氯己定以及溶液的组合，体外。
研究设计：
研究样本包括安装在牙石中的六十颗下颌前磨牙。根管治疗是
在蒸馏水灌溉下使用冠朝下技术制备。样本经过过夜灭菌
环氧乙烷气体。每个根管完全充满粪肠球菌悬浮液并在 37°C 下孵育
24小时。用测试溶液冲洗根管。每次使用体积为 5 ml 的测试冲洗液
样品暴露时间为 5 分钟。所有样品均使用 10 ml 蒸馏水作为最终冲洗液。
在灌溉之前和之后对运河进行采样。系列稀释后，将样品涂在 Mitis salivarius 上
琼脂，并对菌落形成单位进行计数。通过卡方检验、Fisher 精确检验和
单向方差分析，然后进行多范围 Tukey HSD 检验，显着性水平设置为 P < 0.05。
结果和结论：
蒸馏水组菌落数减少量显着低于平均值
所有其他组均减少。然而，没有其他对比具有统计显着性。钠的组合
次氯酸盐和氯己定显示出最有效的抗菌活性，其次是次氯酸钠
和过氧化氢一起。单独使用时，过氧化氢是效果最差的冲洗剂
Objective:
To compare the reduction of E. faecalis counts in root canals produced by irrigation with distilled water,
hydrogen peroxide, sodium hypochlorite, chlorhexidine, and combinations of solutions, in vitro.
Study Design:
Study sample included sixty mandibular premolar teeth mounted in dental stone. Root canals were
prepared using crown down technique under distilled water irrigation. Specimens were sterilized overnight by
ethylene oxide gas. Each canal was completely filled up with the E. faecalis suspension and incubated at 37oC for
24 hours. Root canals were irrigated with the test solutions. A volume of 5 ml of the tested irrigant was used for each
sample with an exposure period of 5 minutes. 10 ml of distilled water was used as final irrigant in all the samples.
Canals were sampled before and after irrigation. After serial dilutions, samples were plated onto Mitis salivarius
agar, and the colony forming units were counted. Results were analyzed by Chi-square test, Fisher’s exact test, and
one-way ANOVA followed by multiple range Tukey HSD test with the level of significance set at P < 0.05.
Results and Conclusions:
Reduction of colony counts in distilled water group was significantly lower than the mean
reduction in all the other groups. However, no other contrasts are statistically significant. Combination of sodium
hypochlorite and chlorhexidine showed the most effective antimicrobial activity followed by sodium hypochlorite
and hydrogen peroxide together. Hydrogen peroxide was the least effective irrigant when used alone