Differential staining of γ synuclein in poorly differentiated compared to highly differentiated colon cancer cells
Differential staining of γ synuclein in poorly differentiated compared to highly differentiated colon cancer cells
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DOI：
10.3892/or.2012.1658
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发表时间：
2012-05-01
期刊：
影响因子：
4.2
通讯作者：
Melzer, Ehud
中科院分区：
文献类型：
--
作者：
Amsterdam, Abraham;Shezen, Elias;Melzer, Ehud
突触核蛋白α、β和γ是通常在神经退行性疾病中发现的蛋白质。然而，有趣的是，突触核蛋白在包括卵巢、乳腺和结肠在内的几种器官的癌细胞中表达。通过免疫细胞化学，使用特异性抗体γ突触核蛋白（SNCG），我们研究了这种蛋白质的分布在低分化，高分化的结肠癌细胞相比。在低分化癌细胞中，肿瘤经常被SNGG抗体强烈染色，表明该蛋白质的高表达。相反，在高度分化的细胞中，没有标记。标记细胞仅见于分化肿瘤细胞的边缘或小叶之间。然而，在中度分化的肿瘤中，SNCG的细胞质染色较弱。有趣的是，在癌症患者（II-IV期）中，低分化和高分化的肿瘤细胞通常存在于同一患者中。用SNCG标记的癌细胞在淋巴结、血管壁周围和脂肪组织中也很明显，其中仅存在低分化癌细胞。由于分化差的癌细胞被认为是具有侵袭性的转移形成，因此建议SNCG可以作为肿瘤细胞用于未分化肿瘤的快速扩散和转移的潜力的标志物。
Synuclein alpha, beta and gamma are proteins usually found in neurodegenerative diseases. However, interestingly synucleins are expressed in cancer cells of several organs including ovary, mammary gland and colon. By immunocytochemistry using specific antibodies to gamma synuclein (SNCG), we examined the distribution of this protein in poorly differentiated, compared to highly differentiated colon cancer cells. In poorly differentiated cancer cells tumors were very frequently stained intensely with antibodies to SNGG, suggesting high expression of this protein. In contrast, in highly differentiated cells, there was no labeling. Labeled cells could be found only at the edges or in between the lobules of the differentiated tumor cells. However, in moderately differentiated tumors, a weak cytoplasmic staining of SNCG was evident. Interestingly in cancer patients (stage II-IV) both poorly and highly differentiated tumor cells were often present in the same patient. Labeled cancer cells with SNCG were evident also in lymph nodes, around the wall of blood vessels and in fat tissue, where only poorly differentiated cancer cells were exclusively present. Since cancer cells with poor differentiation are believed to be aggressive with metastases formation it is suggested that SNCG can serve as a marker for the potential of the tumor cell for the rapid spreading and metastazing of the non-differentiated tumors.