COMPARISON OF LIVE BACTERIA INFUSIONS IN A PORCINE MODEL OF ACUTE RESPIRATORY-FAILURE
COMPARISON OF LIVE BACTERIA INFUSIONS IN A PORCINE MODEL OF ACUTE RESPIRATORY-FAILURE
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DOI：
10.1016/0022-4804(83)90054-9
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
CLOUTIER, CT
中科院分区：
文献类型：
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作者：
DEHRING, DJ;CROCKER, SH;CLOUTIER, CT
脓毒症相关的急性呼吸衰竭（ARF）仍然具有高死亡率和不确定的发病机制。用可重复的铜绿假单胞菌输注活体猪模型，将该生物体的气体交换、血流动力学和肺清除率与活金黄色葡萄球菌和大肠杆菌进行比较。对轻度麻醉的雄性混种猪（15-30 kg）进行插管;使其自主呼吸，并通过切开进行股动脉、中心静脉和Swan-Ganz导管插入术。收集基线数据后，1倍。109个微生物/20 kg/min输注到中心静脉中，持续4 h，并频繁监测变量。立即尸检进行相关的定量组织培养研究。S.金黄色葡萄球菌猪保持了较高的肺细菌清除率，伴肺动脉高压，PO 2 [分压O2]无显著降低，尸检时肺相对正常。铜绿假单胞菌和E. coli动物出现全身性低血压、肺动脉高压、肺血管阻力增加、低氧血症和肺清除率降低。他们的肺部严重充血和水肿。这些研究证实了E.大肠杆菌和铜绿假单胞菌输注猪作为脓毒症诱导的ARF模型在人类中的研究结果还表明，肺动脉高压或肺部细菌清除都不足以引起ARF。
Acute respiratory failure (ARF) related to sepsis continues to have a high mortality and uncertain pathogenesis. With a reproducible live Pseudomonas aeruginosa infusion pig model, the gas exchange, hemodynamics, and pulmonary clearance of this organism were compared with live Staphylococcus aureus and Escherichia coli. Lightly anesthetized, male, mixed-breed pigs, 15-30 kg, were intubated; they were allowed to breathe spontaneously and had femoral artery, central venous and Swan-Ganz catheterization through cutdowns. After baseline data were collected, .apprx. 1 .times. 109 organisms/20 kg/min were infused into a central vein for 4 h with frequent monitoring of the variables. Immediate autopsies were done for related quantitative tissue culture studies. S. aureus pigs maintained a high rate of lung bacterial clearance with pulmonary hypertension, a nonsignificant decrease in PO2 [partial pressure O2], and relatively normal lungs at autopsy. P. aeruginosa and E. coli animals developed systemic hypotension, pulmonary hypertension, increased pulmonary vascular resistance, hypoxemia and decreased pulmonary clearance. Their lungs had gross congestion and edema. The studies confirm the suitability of E. coli and P. aeruginosa infusion into pigs as a model of sepsis-induced ARF in man. The findings also indicate that neither pulmonary hypertension nor bacterial clearance by the lungs is sufficient to cause ARF.