Rapid, selective digestion of mitochondrial DNA in accordance with the matA hierarchy of multiallelic mating types in the mitochondrial inheritance of Physarum polycephalum.
Rapid, selective digestion of mitochondrial DNA in accordance with the matA hierarchy of multiallelic mating types in the mitochondrial inheritance of Physarum polycephalum.
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DOI：
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发表时间：
2003-07
期刊：
影响因子：
3.3
通讯作者：
Y. Moriyama;Shigeyuki Kawano
中科院分区：
文献类型：
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作者：
Y. Moriyama;Shigeyuki Kawano
虽然线粒体在几乎所有的真核生物中都是单亲遗传的，但其机制尚不清楚。当受精卵的同花原生生物多头绒泡菌（Physarumpolycephalum）的DAPI染色时，一半的线粒体中的mtDNA的荧光同时下降，产生小斑点，然后在核融合后约1.5小时完全消失，而其他线粒体类核和所有的线粒体鞘保持不变。单个受精卵细胞的PCR分析证实，缺失仅限于来自一个亲本的mtDNA。交配后60 h，线粒体鞘逐渐消失。利用6种交配类型，在所有可能的杂交中检测mtDNA的传递模式。在60个十字架，严格的单亲遗传证实按照相对性层次结构。然而，在其他杂交中，来自父母双方的mtDNA被传递给疟原虫。双亲mtDNA的比例为1：1 ~ 1：10（-4）。尽管如此，还是遵循了matA层次结构。在这些杂交中，mtDNA被不完全消化，并且mtDNA在随后的原质团发育期间复制。我们的结论是，线粒体DNA的快速，选择性消化促进单亲遗传的线粒体，当这失败，双亲遗传发生。
Although mitochondria are inherited uniparentally in nearly all eukaryotes, the mechanism for this is unclear. When zygotes of the isogamous protist Physarum polycephalum were stained with DAPI, the fluorescence of mtDNA in half of the mitochondria decreased simultaneously to give small spots and then disappeared completely approximately 1.5 hr after nuclear fusion, while the other mitochondrial nucleoids and all of the mitochondrial sheaths remained unchanged. PCR analysis of single zygote cells confirmed that the loss was limited to mtDNA from one parent. The vacant mitochondrial sheaths were gradually eliminated by 60 hr after mating. Using six mating types, the transmission patterns of mtDNA were examined in all possible crosses. In 39 of 60 crosses, strict uniparental inheritance was confirmed in accordance with a hierarchy of relative sexuality. In the other crosses, however, mtDNA from both parents was transmitted to plasmodia. The ratio of parental mtDNA was estimated to be from 1:1 to 1:10(-4). Nevertheless, the matA hierarchy was followed. In these crosses, the mtDNA was incompletely digested, and mtDNA replicated during subsequent plasmodial development. We conclude that the rapid, selective digestion of mtDNA promotes the uniparental inheritance of mitochondria; when this fails, biparental inheritance occurs.