The Storage Form of Human Renal Renin
The Storage Form of Human Renal Renin
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人肾肾素的储存形式
DOI：
10.1161/01.hyp.4.2.211
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发表时间：
1982
期刊：
影响因子：
8.3
通讯作者：
M. Ikeda
中科院分区：
文献类型：
--
作者：
M. Kawamura;S. Akabane;K. Ito;K. Ogino;C. Yutani;S. Go;M. Ikeda
我们用不连续蔗糖密度梯度离心法从尸体肾脏中分离出了肾素颗粒，并研究了这些颗粒中的肾素的贮存形式。约23%的原bomogenate中的总肾素活性是从1.6和1.7 M蔗糖之间的表面相获得的（馏分6）。从组分6中的颗粒提取的颗粒肾素使用胃蛋白酶抑制剂亲和层析分离成活性和非活性的肾素。只有活性肾素对胃酶抑素有亲和力。无活性的肾素，虽然被胰蛋白酶激活，但很少被酸化激活。非活性凝乳酶的比例约占总颗粒凝乳酶（活性凝乳酶+非活性凝乳酶）的25%。当胰蛋白酶浓度超过10 μg/ml时，胰蛋白酶激活的肾素活性降低，而活性肾素的酶活性则被胰蛋白酶抑制。通过凝胶过滤，失活的肾素显示出单峰，分子量（MW）为48，000。活性肾素的分子量为44，000。胰蛋白酶可激活失活的肾素，但分子量无明显变化。（高血压4：211-218，1982年）
We isolated renin granules from cadaver kidneys using discontinuous sucrose density gradient centrifugation, and investigated the storage form of the renin from these granules. Approximately 23% of the total renin activity in the original bomogenate was obtained from the surface phase between 1.6 and 1.7 M sucrose (Fraction 6). Granule renin extracted from the granules in Fraction 6 was separated into active and inactive renin using pepstatin affinity chromatography. Only the active renin had an affinity for pepstatin. The inactive renin, albeit activated by trypsln, was little activated by acidification. The proportion of inactive renin was about 25% of the total granule renin (active renin + inactive renin). Trypsln concentrations over 10 μg/m resulted in a decrease in the renin activity of the trypsln-activated renin, but the enzymatic activity of active renin was decreased by trypsln. With gel filtration, the Inactive renin revealed a single peak, and the molecular weight (MW)was 48,000. The active renin had a MW of 44,000. The inactive renin could be activated by trypsln without an apparent change in molecular weight. (Hypertension 4: 211–218, 1982)