Expression and activity of dehydroepiandrosterone sulfotransferase in human gastric mucosa
Expression and activity of dehydroepiandrosterone sulfotransferase in human gastric mucosa
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DOI：
10.1016/s0960-0760(00)00020-0
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发表时间：
2000-03-01
影响因子：
4.1
通讯作者：
Hongo, M
中科院分区：
文献类型：
--
作者：
Tashiro, A;Sasano, H;Hongo, M
脱氢表雄酮磺转移酶（DHEA-ST）是形成脱氢表雄酮硫酸酯（DHEAS）的关键酶，被认为参与多种物质如胆汁酸和胆固醇的转化，除在成人的肾上腺和肝脏中存在外，在小肠中也存在DHEA-ST。由于硫转移酶可以作用于有毒或潜在有毒物质，降低其生物活性，我们试图阐明DHEA-ST在胃肠道中的意义。我们检查了手术切除的人胃中DHEA-ST的存在并试图确定其可能的生物学意义，7例DHEA-ST的活性范围从6到84 pmol /mg蛋白/90 min不等。免疫印迹显示一个与DHEA-ST分子量相对应的35 kda蛋白单条带。DHEA-ST免疫反应性和mRNA杂交信号均定位于胃腺壁细胞。我们目前的研究结果表明，脱氢表雄酮的磺化脱氢表雄酮发生在胃腺。DHEA-ST在壁细胞中的定位表明，该酶除了与外源性物质解毒外，还与人胃粘膜功能有关。(C) 2000 Elsevier Science Ltd.版权所有。
Dehydroepiandrostorone sulfotransferase (DHEA-ST) is a key enzyme in the formation of Dehydroepiandrosterone sulfate (DHEAS) and is thought to be involved in the conversion of various substances such as bile acids and cholesterol, The existence of DHEA-ST in the small intestine in addition to the adrenal gland and liver in adult humans was recently reported. As the sulfotransferases can act on toxic or potentially toxic substances to reduce their biological activity, we attempted to clarify the significance of DHEA-ST in gastrointestinal tract, We examined surgically resected human stomach for the presence of DHEA-ST and attempted to determine its possible biological significance, DHEA-ST activity ranged widely from 6 to 84 pmoles/mg protein/90 min in 7 cases. Immunoblotting revealed one single band of a 35-kDa protein corresponding to the moleculr weight of DHEA-ST. Both DHEA-ST immunoreactivity and mRNA hybridization signals were localized in parietal cells of the gastric glands. The results of our present study demonstrated that the sulfation of DHEA by DHEA-ST occurs in the gastric glands. The localization of DHEA-ST in parietal cells suggests that this enzyme is correlated to mucosal function in the human stomach in addition to detoxification of exogenous substances. (C) 2000 Elsevier Science Ltd. All rights reserved.