Estrogens and the hypothalamus: nuclear receptor and RNA polymerase activation.
Estrogens and the hypothalamus: nuclear receptor and RNA polymerase activation.
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雌激素和下丘脑：核受体和 RNA 聚合酶激活。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
E. Peck
中科院分区：
文献类型：
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作者：
K. Kelner;Ann L. Miller;E. Peck
为了进一步了解雌激素在中枢神经系统中的作用，我们建立了一种成年大鼠脑内雌激素核受体(RNE)的定量方法。粗染色质通过1.2M蔗糖垫对脑匀浆进行离心法从可溶性和膜性部分中分离出来。将小球与[~3H]雌二醇在高温下孵育，并用硫酸鱼精蛋白沉淀受体类固醇络合物，即可测定RNe。该方法的DNA产率为70-80%，测得的RNE量与DNA的加入量呈线性关系。用此方法测定了去卵巢成年大鼠注射5 ug雌二醇后下丘脑RNe随时间的变化，发现1h时RN值达到峰值，约为30fmol/下丘脑。12h后，RNe水平恢复到对照组(未注射)水平。我们还测量了雌激素诱导下丘脑内源性核糖核酸聚合酶激活的时间进程。
AbstractTo further the understanding of estrogen action in the central nervous system, we have developed a procedure for quantitation of nuclear estrogen receptor (RnE) in adult rat brain. Crude chromatin is separated from soluble and membranous fractions by centrifugation of brain homogenate through 1.2 M sucrose pads. Incubation of the pellet with [3H] estradiol at elevated temperature and precipitation of receptor-steroid complexes with protamine sulfate allows measurement of RnE. The yield of DNA by this procedure is 70–80% and the amount of RnE measured is linear with respect to the amount of DNA added. Using this procedure, we have measured hypothalamic RnE in the ovariectomized adult rat as a function of time after injection of 5 ug estradiol and found a peak RnE accumulation of about 30 fmol/hypothalamus at 1 h. By 12 h, the levels of RnE return to control (uninjected) values.We have also measured the time course of estradiol induced activation of hypothalamic endogenous nuclear RNA polymerases I ...