Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities.
Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities.
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DOI：
10.2147/ijn.s28098
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发表时间：
2012
影响因子：
8
通讯作者：
Wei S
中科院分区：
文献类型：
--
作者：
Liu X;Zhao M;Lu J;Ma J;Wei J;Wei S
羟基磷灰石（HA）是人体骨骼的主要无机成分。由于透明质酸具有良好的生物相容性和骨导电性，因此采用不同的方法制备了各种透明质酸颗粒。许多报道表明，透明质酸的性质影响其生物学效应。采用水热法在不同温度下制备了两种不同尺寸和结晶度的纳米羟基磷灰石。结果发现，在温度为140℃时，生成的棒状晶体（n-HA1）直径为23±5 nm，长度为47±14 nm，结晶度为85%±5%；在温度为80℃时，生成的棒状晶体（n-HA2）直径为16±3 nm，长度为40±10 nm，结晶度为65%±3%。采用MTT、扫描电镜和流式细胞术研究纳米羟基磷灰石大小和结晶度对成骨细胞活力的影响。n-HA1在促进细胞生长和抑制细胞凋亡方面表现出比n-HA2更好的生物学应答，并且表现出比n-HA2更活跃的细胞形态。n-HA2和n-HA1的碱性磷酸酶活性均显著高于对照，n-HA1和n-HA2之间无显著差异。Western blotting检测I型胶原蛋白和骨桥蛋白的表达也有相同的趋势。此外，透射电镜发现大量的n-HA2进入细胞并破坏细胞形态。n-HA2释放的肿瘤坏死因子α明显高于n-HA1，表明n-HA2可能引发严重的炎症反应。这项工作表明，并不是所有的纳米羟基磷灰石都应该被认为是未来临床应用的良好生物材料。
Hydroxyapatite (HA) is the principal inorganic constituent of human bone. Due to its good biocompatibility and osteoconductivity, all kinds of HA particles were prepared by different methods. Numerous reports demonstrated that the properties of HA affected its biological effects. Two kinds of nanohydroxyapatite with different sizes and crystallinities were obtained via a hydrothermal treatment method under different temperatures. It was found that at a temperature of 140°C, a rod-like crystal (n-HA1) with a diameter of 23 ± 5 nm, a length of 47 ± 14 nm, and crystallinity of 85% ± 5% was produced, while at a temperature of 80°C, a rod-like crystal (n-HA2) with a diameter of 16 ± 3 nm, a length of 40 ± 10 nm, and crystallinity of 65% ± 3% was produced. The influence of nanohydroxyapatite size and crystallinity on osteoblast viability was studied by MTT, scanning electron microscopy, and flow cytometry. n-HA1 gave a better biological response than n-HA2 in promoting cell growth and inhibiting cell apoptosis, and also exhibited much more active cell morphology. Alkaline phosphatase activity for both n-HA2 and n-HA1 was obviously higher than for the control, and no significant difference was found between n-HA1 and n-HA2. The same trend was observed on Western blotting for expression of type I collagen and osteopontin. In addition, it was found by transmission electron microscopy that large quantities of n-HA2 entered into the cell and damaged the cellular morphology. Release of tumor necrosis factor alpha from n-HA2 was markedly higher than from n-HA1, indicating that n-HA2 might trigger a severe inflammatory response. This work indicates that not all nanohydroxyapatite should be considered a good biomaterial in future clinical applications.