Molecular characterization and expression analysis of the mulberry Na+/H+ exchanger gene family
Molecular characterization and expression analysis of the mulberry Na+/H+ exchanger gene family
复制标题
DOI：
10.1016/j.plaphy.2015.12.010
复制
发表时间：
2016-02-01
影响因子：
6.5
通讯作者：
Zhao, Aichun
中科院分区：
文献类型：
--
作者：
Cao, Boning;Long, Dingpei;Zhao, Aichun
Na+/H+交换体（NHXs）在植物细胞内pH、Na+和K+的稳态中起着重要作用。桑葚不仅是一种重要的传统经济木本植物，其叶子是家蚕的唯一食物来源，而且它还可以适应许多不同的不利条件，包括盐环境。然而，很少有人知道NHX在这个重要的多年生树木。在这项研究中，我们确定并克隆了七个推定的NHX基因家族成员的Mows atropurpurea的基础上，全基因组分析的Mows基因组数据库。桑葚NHX的系统发育分析和基因组结构表明，桑葚NHX家族形成三个不同的亚群。在正常培养条件下对不同桑葚品种的转录组数据和实时荧光定量PCR结果显示，桑葚NHX家族在两种桑葚中具有不同的组织特异性模式。不同胁迫条件下MaNHX基因的表达分析（盐和干旱）和信号分子（脱落酸、水杨酸、过氧化氢和茉莉酸甲酯）的研究表明，MaNHX不仅可以被文献中描述的盐、干旱和脱落酸诱导，而且还可以被其他信号分子诱导，表明MaNHX成员在不同的非生物胁迫、植物激素和植物信号分子作用下，在桑葚不同组织中表现出多样而复杂的表达模式。我们的研究结果提供了一些见解，这组H+偶联阳离子交换剂的新的和新兴的细胞和生理功能，超出其耐盐性的功能，也提供了基础，进一步表征MaNHX的生理功能。(C)2015年Elsevier Masson SAS。All rights reserved.
Na+/H+ exchangers (NHXs) have important roles in cellular pH, and Na+ and K+ homeostasis in plants. Mulberry is not only an important traditional economic woody plant known for its leaves, which are the exclusive food source of the silkworm Bombyx mori, but it can also adapt to many different adverse conditions, including saline environments. However, little is known about the NHXs in this important perennial tree. In this study, we identified and cloned seven putative NHX gene family members from Mows atropurpurea based on a genome-wide analysis of the Mows genome database. A phylogenetic analysis and genomic organization of mulberry NHXs suggested that the mulberry NHX family forms three distinct subgroups. Transcriptome data and real-time PCR of different mulberry varieties under normal culture conditions revealed that the mulberry NHX family has a different tissue-specific pattern in the two mulberry species. The MaNHX genes' expression analyses under different stresses (salt and drought) and signal molecules (abscisic acid, salicylic acid, hydrogen peroxide and methyl jasmonate) revealed that MaNHXs not only could be induced by salt, drought and abscisic acid as describe in the literature, but were also induced by other signal molecules, which indicated that MaNHX members exhibited diverse and complicated expression patterns in different mulberry tissues under various abiotic stresses, phytohormones and plant signaling molecules. Our results provide some insights into new and emerging cellular and physiological functions of this group of H+-coupled cation exchangers, beyond their function in salt tolerance, and also provide the basis for further characterizations of MaNHXs' physiological functions. (C) 2015 Elsevier Masson SAS. All rights reserved.