Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen
Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen
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DOI：
10.1016/s0093-691x(97)84066-6
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发表时间：
1997-07-15
期刊：
影响因子：
2.8
通讯作者：
Aurich, C
中科院分区：
文献类型：
--
作者：
Aurich, JE;Schonherr, U;Aurich, C
冷冻储存的种马精液的使用受到其相对短期受精能力的限制。精子储存期间生育能力下降的一个重要原因是精子膜脂过氧化。本研究研究了抗坏血酸（0.45和0.9 g/L）和过氧化氢酶(0.45 × 10（6)和1.8 × 10(6)单位/L）对冷藏种马精液的影响。采用人工阴道采集7匹公马的精液，分别用脱脂粉剂、增精剂或甘氨酸增精剂稀释。在5℃下稀释后、24、48和72 h，研究精子活力和膜完整性。与对照组相比，抗坏血酸显著提高了5℃下24、48和72 h的精子膜完整率(P
The use of chilled-stored stallion semen is limited by its relatively short-term fertilizing capacity. An important reason for the decrease in fertility during storage is the peroxidation of sperm membrane lipids. In this study, effects of the antioxidants ascorbic acid (0.45 and 0.9 g/L) and catalase (0.45 x 10(6) and 1.8 x 10(6) units/L) on chilled-stored stallion semen were investigated. Semen was collected by artificial vagina from 7 stallions and was diluted with skim mill: extender or glycin extender. Sperm motility and membrane integrity were investigated after dilution and after 24, 48 and 72 h at 5 degrees C. Ascorbic acid significantly increased the percentage of membrane-intact spermatozoa at 24, 48 and 72 h at 5 degrees C when compared with that of the controls (P