Subunit structure and activation of inactive phosphorylase phosphatase.

Subunit structure and activation of inactive phosphorylase phosphatase.
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DOI:
10.1021/bi00283a014
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发表时间:
1983-07
期刊:
影响因子:
2.9
通讯作者:
L. Ballou;D. Brautigan;E. Fischer
L. Ballou;D. Brautigan;E. Fischer
中科院分区:
生物学3区
文献类型:
--
作者:
L. Ballou;D. Brautigan;E. Fischer

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材料与方法按Fischer & Krebs(1958)的方法纯化兔肌磷酸化酶B。(1968),并根据Cohen(1973)纯化磷酸化酶激酶。牛心脏cAMP依赖性蛋白激酶的催化亚基是通过Peters等的方法分离的。(1977年)。磷酸化酶a如通过以下步骤所述制备:
Materials and MethodsRabbit muscle phosphorylase b was purified according to Fischer & Krebs (1958) as modified by DeLange et al.(1968), and phosphorylase kinase was purified according to Cohen (1973). The catalytic subunit of bovine cardiac cAMP-de-pendent protein kinase was isolated by the method of Peters et al.(1977). Phosphorylase a was prepared as described by