Stimulation of Agrobacterium tumefaciens T-DNA transfer by overdrive depends on a flanking sequence but not on helical position with respect to the border repeat.
Stimulation of Agrobacterium tumefaciens T-DNA transfer by overdrive depends on a flanking sequence but not on helical position with respect to the border repeat.
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通过过驱动对根癌农杆菌 T-DNA 转移的刺激取决于侧翼序列，但不取决于相对于边界重复的螺旋位置。
DOI：
10.1128/jb.173.17.5558-5563.1991
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发表时间：
1991
影响因子：
3.2
通讯作者：
Ream,W
中科院分区：
文献类型：
--
作者：
Shurvinton,CE;Ream,W
根癌农杆菌的T-DNA转移依赖于T-DNA的右边界重复序列，并受到邻近序列的极大刺激。我们报告说，功能不依赖于螺旋位置相对于边界重复。一个合成的24-bp的序列和一个12-bp的区域，包含一个完全保守的8-bp的核心序列刺激毒力相同，但完整的功能需要额外的碱基的24-bp序列的左边。
T-DNA transfer by Agrobacterium tumefaciens depends on the right border repeat of the T-DNA and is greatly stimulated by overdrive, an adjacent sequence. We report that the function of overdrive does not depend on helical position with respect to the border repeat. A synthetic 24-bp overdrive and a 12-bp region containing a fully conserved 8-bp core overdrive sequence stimulated virulence equally, but full function required additional bases to the left of the 24-bp sequence.