Effects of Manisa propolis on telomerase activity in leukemia cells obtained from the bone marrow of leukemia patients
Effects of Manisa propolis on telomerase activity in leukemia cells obtained from the bone marrow of leukemia patients
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DOI：
10.3109/09637480801994712
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发表时间：
2009-01-01
影响因子：
3.9
通讯作者：
Ozkinay, F.
中科院分区：
文献类型：
--
作者：
Cogulu, O.;Biray, C.;Ozkinay, F.
蜂胶是一种由蜜蜂收集的树脂材料，从蜂箱中获得，通过诱导细胞凋亡具有抗癌作用。本研究旨在探讨蜂胶对白血病患者白血病细胞端粒酶逆转录酶（hTERT）的影响。从四个白血病病例中分别制备了四个不同的骨髓细胞培养物。每位患者的3个培养中分别加入60、30和15 ng/ml工作浓度的蜂胶，其中1个培养只含有培养基。在24 h、48 h和72 h用LightCycler 2.0仪器检测细胞hTERT mRNA的表达水平。蜂胶浓度为60 ng/ml时，hTERT表达量显著降低。综上所述，马尼沙蜂胶也可能对白血病中hTERT的表达有潜在的影响，特别是由于它的成分菊素。
Propolis is a resinous material collected by honeybees and obtained from beehives that has anticancer effects by inducing apoptosis. The aim of this study is to investigate the effect of propolis on human telomerase reverse transcriptase (hTERT) in the leukemia cells obtained from leukemia patients. Four different bone marrow cell cultures from each of four leukemia cases were prepared. The 60 ng/ml, 30 ng/ml and 15 ng/ml working concentrations of propolis were administered to three cultures of each patient, while one culture contained only culture medium. hTERT mRNA expression levels of cells were detected at 24 h, 48 h and 72 h using the LightCycler 2.0 instrument. A significant decrease in hTERT expression levels was observed in the 60 ng/ml concentration of propolis. In conclusion, Manisa propolis may also have a potential effect on the expression of hTERT in leukemia-particularly owing to its constituent chrysin.