Improvement of monolithic solid material by utilization of spacer for identification of the target using affinity resins
Improvement of monolithic solid material by utilization of spacer for identification of the target using affinity resins
复制标题
DOI：
10.1016/j.bmcl.2009.01.050
复制
发表时间：
2009-03-01
影响因子：
2.7
通讯作者：
Tanaka, Akito
中科院分区：
文献类型：
--
作者：
Iwaoka, Emiko;Mori, Tomoko;Tanaka, Akito
亲和色谱法是鉴定靶标的重要手段。然而，商业上可用的固体材料有局限性，而选择固体材料有时是至关重要的。我们在以前的论文中报道了一种具有整体结构的合成树脂。本文以苯磺酰胺为诱饵，定量分析了隔离剂引入整体材料对特异性结合蛋白鉴定的影响，表明引入x-取代庚酸作为隔离剂使亲和树脂能够有效捕获目标蛋白。利用优化后的间隔剂，承载FK506的单片材料不仅可以在银染色水平上识别FKBP12，还可以识别FKBP52、钙调神经磷酸酶A和钙调神经磷酸酶B，而没有间隔剂的单片材料则无法识别。2009爱思唯尔有限公司版权所有。
Affinity chromatography is an important strategy for target identifications. However, commercial available solid materials have limitations while selection of that is sometimes vital for the purpose. We have reported a synthetic resin with a monolithic structure in previous papers. In this paper, introduction effects of spacer to the monolithic material on identification of specific binding protein was quantitatively analyzed using benzensulfonamide as a bait, which exhibited introduction of x-substituted heptanoic acid as spacer enabled affinity resins to capture the target proteins effectively. Utilization of the optimized spacer enable the monolithic material bearing FK506 to identify not only FKBP12 but FKBP52, calcineurin A and calcineurin B at silver stained level, while that without spacer had failed. (C) 2009 Elsevier Ltd. All rights reserved.