MEF2-mediated recruitment of class 11 HDAC at the EBV immediate early gene BZLF1 links latency and chromatin remodeling
MEF2-mediated recruitment of class 11 HDAC at the EBV immediate early gene BZLF1 links latency and chromatin remodeling
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DOI：
10.1093/embo-reports/kvf031
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发表时间：
2002-02-01
期刊：
影响因子：
7.7
通讯作者：
Sergeant, A
中科院分区：
文献类型：
--
作者：
Gruffat, H;Manet, E;Sergeant, A
在体外由EB病毒（EBV）诱导增殖的B淋巴细胞中，包装在染色质中的染色体外病毒游离体持续存在于细胞核中，并且没有生产性循环。在诱导表达EBV BZLF1基因产物（转录因子EB1）后，观察到从该潜伏期到生产周期的转换。我们目前的证据表明，在潜伏期，肌细胞增强子结合因子2（MEF2）家族的蛋白质绑定到BZLF1启动子和招聘II类组蛋白脱乙酰酶。此外，我们提出，潜伏期主要是由MEF2D的BZLF1基因启动子的II类组蛋白脱乙酰酶（HDAC）的特异性和本地招聘。从潜伏期到生产周期的转变可能部分是由于MEF2蛋白的翻译后修饰和染色质局部乙酰化状态的变化。
In B lymphocytes induced to proliferate in vitro by the Epstein-Barr virus (EBV), extra-chromosomal viral episomes packaged in chromatin persist in the nucleus, and there is no productive cycle. A switch from this latency to the productive cycle is observed after induced expression of the EBV BZLF1 gene product, the transcription factor EB1. We present evidence that, during latency, proteins of the myocyte enhancer binding factor 2 (MEF2) family are bound to the BZLF1 promoter and recruit class II histone deacetylases. Furthermore, we propose that latency is determined primarily by a specific and local recruitment of class II histone deacetylase (HDAC) by MEF2D to the BZLF1 gene promoter. The switch from latency to the productive cycle could be due in part to post-translational modification of MEF2 proteins and changes in the local acetylation state of the chromatin.