Role of cathepsins and cystatins in patients with recurrent miscarriage
Role of cathepsins and cystatins in patients with recurrent miscarriage
复制标题
DOI：
10.1093/molehr/gah172
复制
发表时间：
2005-05-01
影响因子：
4
通讯作者：
Suzumori, K
中科院分区：
文献类型：
--
作者：
Nakanishi, T;Ozaki, Y;Suzumori, K
在着床过程中，滋养细胞通过分泌蛋白酶穿透母体蜕膜。据报道，组织蛋白酶在小鼠绒毛中高度表达，在正常胚胎生长和脱体细胞化过程中起重要作用。在这项研究中，我们评估了组织蛋白酶及其内源性抑制剂胱抑素在复发性流产患者组织和血清中的含量。22例患者和12例健康妇女手术采集蜕膜和绒毛。免疫组化检测组织蛋白酶、胱抑素A（胱抑素A）、胱抑素B（胱抑素B）和胱抑素C抗体。酶联免疫吸附法检测组织蛋白酶、胱抑素A和胱抑素C的浓度。此外，我们测量了85名日本复发性流产妇女血清胱抑素C水平。蜕膜上皮细胞细胞质中组织蛋白酶B、D、H、L、stein B和胱抑素C染色。Stefin A在滋养细胞表面表达。患者蜕膜组织蛋白酶B和H浓度明显高于对照组。患者血清胱抑素C水平明显低于对照组。我们的研究结果表明，组织蛋白酶-胱抑素系统的调节可能在复发性流产患者中起重要作用。
In the implantation, trophoblasts penetrate maternal decidua by secreting proteases. It has been reported that cathepsins are highly expressed in the mouse villi, and play an important role in normal embryonal growth and decidualization. In this study, we evaluated cathepsins and their endogenous inhibitors, cystatins, in tissue and serum of patients with recurrent miscarriage. Decidua and villi were surgically collected from 22 patients and 12 healthy women. Immunohistochemistry was performed with antibodies against cathepsins, stefin A (cystatin A), stefin B (cystatin B) and cystatin C. The concentrations of cathepsins, stefins and cystatin C were measured by Enzyme-linked immunosorbent assay. In addition, we measured the serum level of cystatin C in 85 Japanese women with recurrent miscarriage. Staining of cathepsin B, D, H, L, stefin B and cystatin C was observed in the cytoplasm of epithelial cells in decidua. Stefin A was expressed on the surface of the trophoblast. The concentration of cathepsin B and H in patients' decidua was significantly higher than in control individuals. The serum level of cystatin C was significantly lower in patients than in control individuals. Our findings suggest that the regulation of the cathepsin-cystatin system may play an important role in patients with recurrent miscarriage.