Delayed intralesional transplantation of bone marrow stromal cells increases endogenous neurogenesis and promotes functional recovery after severe traumatic brain injury
Delayed intralesional transplantation of bone marrow stromal cells increases endogenous neurogenesis and promotes functional recovery after severe traumatic brain injury
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DOI：
10.1080/02699050903133970
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发表时间：
2009-01-01
期刊：
影响因子：
1.9
通讯作者：
Vaquero, Jesus
中科院分区：
文献类型：
--
作者：
Bonilla, Celia;Zurita, Mercedes;Vaquero, Jesus
主要目标：目的探讨骨髓基质细胞（BMSC）延迟移植对脑外伤后神经系统后遗症的改善作用。研究方法：成年Wistar大鼠进行了重量下降的影响，造成严重的脑损伤，2个月后，在盐水中的BMSC，或盐水单独注射到受伤的脑组织。在接下来的两个月里，两个实验组都通过旋转棒和改良的神经系统严重程度评分（mNSS）测试进行了评估。此时，处死动物，通过组织学和免疫组织化学技术研究其脑。结果：与对照组相比，转棒试验和mNSS试验显示BMSC移植大鼠的功能恢复进行性.移植后2个月，BMSC在宿主组织中存活，部分BMSC表达Neu-N或GFAP，提示神经元和星形胶质细胞转分化。此外，与对照组相比，BMSC移植大鼠的内源性神经发生显著增加。结论：这些发现提示延迟脑内移植骨髓间充质干细胞治疗创伤性脑损伤后遗症的实用性。
Primary objective: To investigate the utility of delayed transplantation of bone marrow stromal cells (BMSC) to improve the neurological sequels after traumatic brain injury (TBI). Methods: Adult Wistar rats were subjected to weight-drop impact causing severe brain injury, and 2 months later, BMSC in saline, or saline alone, were injected into injured brain tissue. Both experimental groups were evaluated by means of rotarod and modified neurologic severity scores (mNSS) tests in the course of the two following months. At this time, the animal were sacrificed and their brains were studied by means of histological and immunohistochemical techniques. Results: Rotarod and mNSS tests showed progressive functional recovery in the BMSC- transplanted rats, compared with controls. Two months after transplantation, BMSC survived in the host tissue, and some of them showed expression of Neu-N or GFAP, suggesting neuronal and astroglial transdifferentiation. Furthermore, significant increase of endogenous neurogenesis was found in BMSC-transplanted rats, compared with controls. Conclusions: These findings suggest the utility of delayed intracerebral transplantation of BMSC for the treatment of established sequels after TBI.