Localization of chemotactic peptide receptors on rabbit neutrophils.
Localization of chemotactic peptide receptors on rabbit neutrophils.
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兔中性粒细胞上趋化肽受体的定位。
DOI：
10.1016/0014-4827(84)90186-1
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发表时间：
1984
影响因子：
3.7
通讯作者：
W. Marasco
中科院分区：
文献类型：
--
作者：
R. Walter;W. Marasco
血液白细胞的趋化性是通过化学引诱物与白细胞表面上的特异性受体结合而启动的[1，2]。尽管对伴随趋化性激活的生物化学和形态学事件有很多了解[3-9]，但关于趋化受体本身的形态学信息很少。后者的信息是必要的，以便我们可以理解这些炎症细胞检测和响应化学梯度的机制。广泛用于表征白细胞活化后的复杂行为反应的一类趋化因子是合成的甲酰甲硫氨酰肽。这些肽，现在已知是由细菌产生的天然存在的N-末端肽的类似物[10]，被释放到培养基中，并且被认为至少部分地负责白细胞在细菌感染部位的积累[1，2]。我们用光学显微镜放射自显影术在家兔腹膜中性粒细胞（PMN）上定位了趋化性六肽N-甲酰正亮氨酰-亮氨酰-苯丙氨酸-正亮氨酰-[125 I]酪氨酰-赖氨酸[N-fNle-Leu-Phe-Nle-[125 I]Tyr-Lys]的受体。通过这种方法，在4 °C孵育的细胞上固有的甲酰肽受体分布在圆形和结构极化的PMN表面上似乎是均匀的。在短暂的37 °C孵育后，细胞在细胞表面或细胞表面附近保留了大部分标记的六肽，此外，极化的PMN将六肽重新分配到远离细胞尾足的前方。
The chemotaxis of blood leukocytes is initiated by the binding of a chemoattractant to specific receptors on the leukocyte cell surface [1, 2]. Although a great deal is known about the biochemical and morphological events accompanying chemotactic activation [3–9], there is very little morphological information about the chemoattractant receptors themselves. This latter information is needed so that we may understand the mechanism by which these inflammatory cells detect and respond to chemical gradients. One class of chemotactic factors extensively used to characterize the complex behavioral responses following leukocyte activation are the synthetic formylmethionyl peptides. These peptides, now known to be the analogs of the naturally occurringN-terminal peptides produced by bacteria [10], are released into culture medium and are believed to be responsible, at least in part, for the accumulation of leukocytes at the sites of bacterial infection [1, 2]. We have localized the receptors for the chemotactic hexapeptideN-formylnorleucyl-leucyl-phenylalanine-norleucyl-[125I]tyrosyl-lysine [N-fNleLeuPheNle[125I]TyrLys] on whole rabbit peritoneal neutrophils (PMN) using light microscope autoradiography. By this method, the inherent formylpeptide receptor distribution on cells incubated at 4 °C appears to be uniform over the surface of both rounded and structurally polarized PMN. Following a short 37 °C incubation, cells retain a large proportion of labelled hexapeptide at or near the cell surface and, in addition, polarized PMN redistribute the hexapeptide anteriorly away from the cell uropod.
DOI：
10.1016/s0021-9258(18)91029-x
发表时间：
1984-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wayne;MarascoS;S. Phan;Henry KrutzschQ;Henry;Showell;Douglas E. FeltnerS;Roderick Nairnll;E. Becker;Peter;Ward
通讯作者：
Wayne;MarascoS;S. Phan;Henry KrutzschQ;Henry;Showell;Douglas E. FeltnerS;Roderick Nairnll;E. Becker;Peter;Ward
N-甲酰化趋化肽在受刺激的人粒细胞中的命运。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jesaitis,AJ;Naemura,JR;Painter,RG;Sklar,LA;Cochrane,CG
通讯作者：
Cochrane,CG