Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes.
Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes.
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DOI：
10.1016/j.brainres.2015.07.048
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发表时间：
2016-05-01
期刊：
影响因子：
2.9
通讯作者：
Zeng, Xianmin
中科院分区：
文献类型：
--
作者：
Pei, Ying;Peng, Jun;Behl, Mamta;Sipes, Nisha S.;Shockley, Keith R.;Rao, Mahendra S.;Tice, Raymond R.;Zeng, Xianmin
关键词：
Induced pluripotent stem cells (iPSC) and their differentiated derivatives offer a unique source of human primary cells for toxicity screens. Here, we report on the comparative cytotoxicity of 80 compounds (neurotoxicants, developmental neurotoxicants, environmental compounds) in iPSC as well as isogenic iPSC-derived neural stem cells (NSC), neurons, and astrocytes. All compounds were tested over a 24-hour period at 10 and 100 μM, in duplicate, with cytotoxicity measured using the MTT assay. Of the 80 compounds tested, 50 induced significant cytotoxicity in at least one cell type; per cell type, 32, 38, 46, and 41 induced significant cytotoxicity in iPSC, NSC, neurons, and astrocytes, respectively. Four compounds (valinomycin, 3,3′,5,5′-tetrabromobisphenol, deltamethrin, triphenyl phosphate) were cytotoxic in all four cell types. Retesting these compounds at 1, 10, and 100 μM using the same exposure protocol yielded consistent results as compared with the primary screen. Using rotenone, we extended the testing to seven additional iPSC lines of both genders; no substantial difference in the extent of cytotoxicity was detected among the cell lines. Finally, the cytotoxicity assay was simplified by measuring luciferase activity using lineage-specific luciferase reporter iPSC lines which were generated from the parental iPSC line.
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DOI：
10.1016/s1387-2656(05)11004-7
发表时间：
2005-01-01
期刊：
BIOTECHNOLOGY ANNUAL REVIEW, VOL 11
影响因子：
--
作者：
Berridge, MV;Herst, PM;Tan, AS
通讯作者：
Tan, AS
影响因子：
2.9
作者：
Carloni, Manuel;Nasuti, Cinzia;Gabbianelli, Rosita
通讯作者：
Gabbianelli, Rosita
影响因子：
5.1
作者：
BACHMANN, MO;DEBEER, Z;MYERS, JE
通讯作者：
MYERS, JE
影响因子：
3.3
作者：
Chaudhary, S.;Parvez, S.
通讯作者：
Parvez, S.
影响因子：
3.8
作者：
Axelstad, Marta;Hansen, Pernille Reimar;Hass, Ulla
通讯作者：
Hass, Ulla