Single and dual hormone secretors in GH3 cultures respond differently to hypothalamic factors.
Single and dual hormone secretors in GH3 cultures respond differently to hypothalamic factors.
复制标题
GH3 培养物中的单一和双重激素分泌者对下丘脑因素的反应不同。
DOI：
10.1016/0303-7207(92)90227-w
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发表时间：
1992
影响因子：
4.1
通讯作者：
Boockfor,FR
中科院分区：
文献类型：
--
作者：
Mogg,RJ;Boockfor,FR
最近对正常和肿瘤垂体细胞培养的研究表明，分泌生长激素（GH）和催乳素（PRL）的细胞含有释放其中一种或两种激素的细胞。为了确定这两种细胞类型是否能受到下丘脑因子的差异调节，我们采用斑块测定法对GH和PRL进行了以下研究。利用含有这两种细胞类型的大鼠肿瘤细胞系GH3细胞的培养，我们发现下丘脑因子血管活性肠肽（VIP）和促甲状腺激素释放激素（TRH）一起使用时比单独使用时对斑块形成的影响更大。这表明细胞在培养中对一种肽有反应，而对另一种没有反应。经雌二醇处理的培养物（只含有双分泌细胞）对VIP和TRH的反应性进行了评估，发现对TRH有反应，而对VIP没有反应。最后，我们评估了暴露于VIP缀合物和蓖麻毒素a链（一种强效细胞毒素）的培养物的肽敏感性。除了消除vip应答细胞外，这种治疗还显著降低了仅分泌gh的细胞比例，而对双激素分泌没有明显影响。综上所述，我们的研究结果表明，单分泌和双分泌对至少两种下丘脑分泌物的反应不同，这表明这些细胞类型之间的调节差异可能对GH和PRL分泌的控制很重要。
Recent studies using both normal and tumoral pituitary cell cultures have demonstrated that growth hormone (GH) and prolactin (PRL) secreting populations contain cells which release either one or both of these hormones. In order to determine whether these two cell types can be differentially regulated by hypothalamic factors we performed the following study employing plaque assays for GH and PRL. Using cultures of GH3 cells, a rat tumor cell line which contains both of these cell types, we found that the hypothalamic factors vasoactive intestinal peptide (VIP) and thyrotropin releasing hormone (TRH) when used together had a greater influence on plaque formation than when each was used individually. This suggested that cells were present in culture that responded to one peptide but not the other. Estradioltreated cultures (which contain only dual-secreting cells) were then evaluated for VIP and TRH responsiveness and found to respond to TRH but not VIP. Finally, we assessed the peptide sensitivity of cultures that were exposed to a conjugate of VIP and the A-chain of ricin (a potent cytotoxin). In addition to eliminating VIP-responsive cells, this treatment markedly reduced the proportions of cells secreting GH-only while having no appreciable influence on dual-hormone secretors. When taken together, our findings indicate that single and dual secretors respond differently to at least two hypothalamic secretagogues and suggest that regulatory differences between these cell types may be important in the control of GH and PRL secretion.