Influence of intracellular trehalose concentration and pre-freeze cell volume on the cryosurvival of rapidly frozen human erythrocytes
Influence of intracellular trehalose concentration and pre-freeze cell volume on the cryosurvival of rapidly frozen human erythrocytes
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DOI：
10.1016/j.cryobiol.2011.04.005
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发表时间：
2011-08-01
期刊：
影响因子：
2.7
通讯作者：
Slater, Nigel K. H.
中科院分区：
文献类型：
--
作者：
Lynch, Andrew L.;Slater, Nigel K. H.
海藻糖对磷脂双分子层具有低渗透性，其作为哺乳动物细胞的无毒细胞内冷冻保存剂的应用引起了人们的极大兴趣。使用膜透化聚合物PP-50将8 +/- 3 mM和266 +/- 22 mM之间的海藻糖引入人红细胞中，可以研究细胞内海藻糖浓度、冷冻前细胞体积和冷冻存活率之间的关系。细胞冷冻存活率随着冷冻前细胞体积增加至新鲜细胞的正常体积而近似线性地增加;在某些情况下，甚至在>100 mM的细胞内海藻糖浓度下，细胞存活率降低与低于正常的冷冻前细胞体积相关。在具有接近正常细胞体积的细胞中摄取>200 mM海藻糖促进细胞冷冻存活提高高达15 +/-5%。(C)2011 Elsevier Inc. All rights reserved.
Significant interest exists in the application of trehalose, which has low permeability to the phospholipid bilayer, as a non-toxic intracellular cryopreservative for mammalian cells. Introduction of between 8 +/- 3 mM and 266 +/- 22 mM trehalose into human erythrocytes using the membrane permeabilizing polymer PP-50 allowed investigation of the relationship between intracellular trehalose concentration, pre-freeze cell volume, and cryosurvival. Cellular cryosurvival increased approximately linearly with pre-freeze cell volume up to the normal volume of fresh cells; diminished cell survival correlated with subnormal pre-freeze cell volume in some cases even at >100 mM intracellular trehalose concentration. Uptake of >200 mM trehalose in cells with near-normal cell volume facilitated enhancement of cellular cryosurvival by up to 15 +/- 5%. (C) 2011 Elsevier Inc. All rights reserved.