Integrating the molecular and cellular basis of odor coding in the Drosophila antenna
Integrating the molecular and cellular basis of odor coding in the Drosophila antenna
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DOI：
10.1016/s0896-6273(03)00094-1
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发表时间：
2003-03-06
期刊：
影响因子：
16.2
通讯作者：
Carlson, JR
中科院分区：
文献类型：
--
作者：
Dobritsa, AA;van der Goes van Naters, W;Carlson, JR
我们调查如何果蝇嗅觉系统的分子和细胞地图的整合。在个体气味受体、神经元和气味之间建立了对应关系。我们描述的Or22a和Or22b受体基因的表达，显示本地化的树突状细胞膜，并找到性二态性。Or22a映射到ab3A神经元，其对丁酸乙酯作出反应。缺失突变体缺乏Or22 a的分析，沿着与转基因救援实验，证实了映射，并证明了一个或基因是所需的嗅觉功能在体内。Or47a在突变细胞中的异位表达鉴定了它所来源的神经元及其气味配体。在野生型细胞中的异位表达表明两种受体可以在单个细胞中起作用。ab3A神经元不依赖于正常的气味受体基因表达来导航到CNS中的靶点。
We investigate how the molecular and cellular maps of the Drosophila olfactory system are integrated. A correspondence is established between individual odor receptors, neurons, and odors. We describe the expression of the Or22a and Or22b receptor genes, show localization to dendritic membranes, and find sexual dimorphism. Or22a maps to the ab3A neuron, which responds to ethyl butyrate. Analysis of a deletion mutant lacking Or22a, along with transgenic rescue experiments, confirms the mapping and demonstrates that an Or gene is required for olfactory function in vivo. Ectopic expression of Or47a in a mutant cell identifies the neuron from which it derives and its odor ligands. Ectopic expression in a wild-type cell shows that two receptors can function in a single cell. The ab3A neuron does not depend on normal odor receptor gene expression to navigate to its target in the CNS.