Effect of homologous placental lactogens, prolactins, and growth hormones on islet B-cell division and insulin secretion in rat, mouse, and human islets: implication for placental lactogen regulation of islet function during pregnancy.
Effect of homologous placental lactogens, prolactins, and growth hormones on islet B-cell division and insulin secretion in rat, mouse, and human islets: implication for placental lactogen regulation of islet function during pregnancy.
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DOI：
10.1210/endo.132.2.8425500
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发表时间：
1993-02
期刊：
影响因子：
4.8
通讯作者：
T. Brelje;D W Scharp;P E Lacy;L. Ogren;F. Talamantes;M. Robertson;H. G. Friesen;R. L. Sorenson
中科院分区：
文献类型：
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作者：
T. Brelje;D W Scharp;P E Lacy;L. Ogren;F. Talamantes;M. Robertson;H. G. Friesen;R. L. Sorenson
母体胰岛功能的上调对于适应怀孕期间对胰岛素需求的增加是至关重要的。在此之前，我们认为产乳活动调节妊娠期间的胰岛功能。然而，这一假设是基于同源PRL对胰岛的影响，因为同源胎盘催乳素(或胰岛)是不可用的。在这项研究中，我们研究了同源胎盘催乳素(PL)、催乳素和生长激素在体外对大鼠、小鼠和人类胰岛的胰岛素分泌和B细胞分裂的直接影响。新生大鼠胰岛在含0-1000 ng/ml大鼠磷脂酶I(RPL-I)、rPRL或RGH的条件下培养8天。每日更换培养液，测定胰岛素浓度。RPL-I和rPRL(500 ng/ml)处理后，胰岛素分泌增加2倍。RGH(1000 ng/ml)可使胰岛素分泌增加30%。同样，在RPL-I和rPRL的存在下，细胞复制，如BrdU掺入B细胞所示，增加了4倍。RPL-I对胰岛素分泌和5‘-溴-2’-脱氧尿苷(BrdU)掺入的ED50分别为70 ng/ml和150 ng/ml。同样，在成年大鼠的胰岛中，胰岛素分泌增加了1.6倍，B细胞复制增加了3倍。新生小鼠胰岛在含500 ng/ml小鼠PL-I、MPL-II、MPRL或MGH的培养液中培养8天。MPL-I、MPL-II和MPRL治疗导致胰岛素分泌增加2倍。MGH诱导的胰岛素分泌增加30%。在MPL-I和MPRL的存在下，BrdU掺入B细胞的量增加3倍，在MPL-II的存在下，BrdU的掺入增加2倍。成年人胰岛在1微克/毫升人(H)PL、hPRL或hGH的存在下培养8天。从6个雌性胰岛分离的人胰岛，hPL(138+/-10%)、hPRL(133+/-9%)和hGH(117+/-3%)与对照胰岛相比显著增加胰岛素分泌。本研究比较了同源PLS、PRLS和GHS对大鼠、小鼠和人胰岛胰岛素分泌和B细胞分裂的直接影响。结果表明，胎盘催乳素直接调节几个物种的胰岛功能，可能是正常妊娠期间胰岛功能增强的主要激素。
Up-regulation of maternal islet function is essential to accommodate the increased demand for insulin during pregnancy. Previously, we suggested that lactogenic activity regulates islet function during pregnancy. However, this hypothesis was based on the effect of homologous PRLs on islets, since the homologous placental lactogens (or islets) were unavailable. In this study we examine the direct effects of homologous placental lactogens (PL), PRL, and GH on insulin secretion and B-cell division in rat, mouse, and human islets in vitro. Neonatal rat islets were cultured for 8 days in the presence of 0-1000 ng/ml rat PL-I (rPL-I), rPRL, or rGH. Media were changed daily, and the insulin concentration was determined. rPL-I and rPRL (500 ng/ml) treatment resulted in a 2-fold increase in insulin secretion. rGH (1000 ng/ml) elicited a 30% increase in insulin secretion. Similarly, cell replication, as indicated by BrdU incorporation into B-cells, was increased 4-fold in the presence of rPL-I and rPRL. The ED50 for insulin secretion and 5'-bromo-2'-deoxyuridine (BrdU) incorporation was 70 ng/ml for rPL-I and 150 ng/ml for rPRL. Similarly, in adult rat islets, insulin secretion was increased 1.6-fold, and B-cell replication increased 3-fold in the presence of the lactogenic hormones. Neonatal mouse islets were cultured for 8 days in the presence of 500 ng/ml mouse (m) PL-I, mPL-II, mPRL, or mGH. mPL-I, mPL-II, and mPRL treatment resulted in a 2-fold increase in insulin secretion. mGH elicited a 30% increase in insulin secretion. BrdU incorporation into B-cells was increased 3-fold in the presence of mPL-I and mPRL and 2-fold in the presence of mPL-II. Adult human islets were cultured for 8 days in the presence of 1 microgram/ml human (h) PL, hPRL, or hGH. For human islets isolated from six pancreata obtained from females, hPL (138 +/- 10%), hPRL (133 +/- 9%), and hGH (117 +/- 3%) significantly increased insulin secretion compared to that from control islets. This study compares the direct effects among homologous PLs, PRLs, and GHs on insulin secretion and B-cell division in rat, mouse, and human islets. The results indicate that placental lactogen directly regulates islet function in several species and is probably the principal hormone responsible for the increased islet function observed during normal pregnancy.