Salmonella typhimurium infection in calves: delayed specific skin reactions directed against the O-antigenic polysaccharide chain
Salmonella typhimurium infection in calves: delayed specific skin reactions directed against the O-antigenic polysaccharide chain
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犊牛鼠伤寒沙门氏菌感染：针对 O 抗原多糖链的延迟特异性皮肤反应
DOI：
10.1128/iai.37.2.737-748.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
A. Lindberg
中科院分区：
文献类型：
--
作者：
J. Robertsson;S. Svenson;A. Lindberg
经口感染鼠伤寒沙门氏菌的12周龄犊牛皮内注射鼠伤寒沙门氏菌脂多糖后出现特异性迟发性皮肤反应。鼠伤寒沙门氏菌5周前。未感染小牛未出现延迟皮肤反应。皮肤活检显示，在感染小牛的皮肤中有大量的单核细胞浸润，但在未感染小牛的皮肤中没有。通过分离出S.鼠伤寒沙门氏菌的粪便标本。延迟的皮肤反应可以被证明是特异性的，并且针对脂多糖的O-多糖链，因为没有脂多糖的脂多糖链，因为没有来自S的粗糙突变体的脂多糖制剂。鼠伤寒沙门氏菌，两个菌株具有不同的O-多糖链，或脂质A引起皮肤反应。为了引起反应，O-多糖必须在大分子复合物中，因为只有在注射由1，2，3，4-二环氧丁烷交联的O-多糖链或来自共价连接到直链12-碳脂肪链形成人工糖脂的O链的八糖之后才能看到皮肤肿胀。注射O-多糖链的纯八糖未能引起迟发性皮肤反应。
Specific delayed skin reactions developed after intradermal injection of Salmonella typhimurium lipopolysaccharide in 12-week-old calves which had been orally infected with S. typhimurium 5 weeks earlier. Uninfected calves showed no delayed skin reactions. Skin biopsies from skin swellings showed a massive infiltration of mononuclear cells in the skin of infected calves but not in those of uninfected calves. Persistence of infection in infected calves was confirmed by isolation of S. typhimurium from fecal specimens. The delayed skin reactions could be shown to be specific and directed against the O-polysaccharide chain of the lipopolysaccharide since none of the lipopolysaccharide chain of the lipopolysaccharide since none of the lipopolysaccharide preparations from a rough mutant of S. typhimurium, two strains with different O-polysaccharide chains, or lipid A elicited skin reactivity. To cause a reaction, the O-polysaccharide had to be in a macromolecular complex, since skin swellings were seen only after injections of either O-polysaccharide chains cross-linked by 1,2,3,4-diepoxybutane or octasaccharides from the O chain covalently linked to a straight 12-carbon aliphatic chain forming an artificial glycolipid. Injection of the pure octasaccharides of O-polysaccharide chains failed to elicit delayed skin reactions.