Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
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DOI：
10.1007/978-1-4939-3043-2_17
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Krey, Thomas
中科院分区：
文献类型：
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作者：
Backovic, Marija;Krey, Thomas
在过去的几十年中，重组蛋白生产已经成为各种研究方向和生物技术应用不可或缺的工具。在众多报道的表达系统中，昆虫细胞提供了产生需要翻译后修饰的复杂靶蛋白的可能性。在果蝇S2细胞中的稳定表达代表了广泛使用的杆状病毒表达系统的有吸引力的替代方案，特别是对于难以表达的蛋白质，例如，膜蛋白或通过复杂的二硫键模式稳定的高度糖基化的多结构域蛋白。在这里，我们提出了所需的稳定的果蝇S2细胞转染子的产生和使用这些转染子的重组蛋白质的生产的方法。
Recombinant protein production has become an indispensable tool for various research directions and biotechnological applications in the past decades. Among the numerous reported expression systems, insect cells provide the possibility to produce complex target proteins that require posttranslational modifications. Stable expression in Drosophila S2 cells represents an attractive alternative to the widely used baculovirus expression system, offering important advantages in particular for difficult-to-express proteins, e.g., membrane proteins or heavily glycosylated multi-domain proteins that are stabilized by a complex disulfide pattern. Here we present the methodology that is required for the generation of stable Drosophila S2 cell transfectants and for production of recombinant proteins using those transfectants.