CXCR5CD4 follicular helper T cells accumulate in resting human lymph nodes and have superior B cell helper activity
CXCR5CD4 follicular helper T cells accumulate in resting human lymph nodes and have superior B cell helper activity
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DOI：
10.1093/intimm/dxt058
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发表时间：
2014-03-01
影响因子：
4.4
通讯作者：
van Lier, Rene A. W.
中科院分区：
文献类型：
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作者：
Havenith, Simone H. C.;Remmerswaal, Ester B. M.;van Lier, Rene A. W.
更多的T-fh细胞，但较少的细胞毒性CD 4 T细胞，存在于静息淋巴结。虽然许多相关的免疫反应是在淋巴结中启动的，但这一区室尚未在人类中进行系统研究。已经对来自扁桃体的免疫细胞进行了分析，但由于该组织最常发炎，因此这些数据的推广是困难的。在这里，我们分析了成对的静息淋巴结和外周血样本中人CD 4 T细胞亚群和谱系的表型和功能。两个隔室中均相等地代表了幼稚、中枢记忆细胞和效应记忆细胞以及T（h）1、T（h）2、T（h）17和Treg细胞。另一方面，淋巴结中明显不存在细胞毒性CD 4 T细胞。CXCR 5 CD 4 T细胞，代表假定的滤泡T-h（Tfh）细胞在淋巴结中过度表达，并表达比其外周血对应物更高水平的Tfh标志物。与循环池相比，淋巴结来源的CXCR 5 CD 4 T细胞在为B细胞提供帮助方面具有上级优势。因此，功能上有能力的Tfh细胞在静息的人淋巴结中积累，在抗原攻击后迅速诱导幼稚和记忆抗体应答。
More T-fh cells, but fewer cytotoxic CD4 T cells, are present in resting lymph nodes.Although many relevant immune reactions are initiated in the lymph nodes, this compartment has not been systematically studied in humans. Analyses have been performed on immune cells derived from tonsils, but as this tissue is most often inflamed, generalization of these data is difficult. Here, we analyzed the phenotype and function of the human CD4 T-cell subsets and lineages in paired resting lymph node and peripheral blood samples. Naive, central memory cells and effector memory cells as well as T(h)1, T(h)2, T(h)17 and Treg cells were equally represented in both compartments. On the other hand, cytotoxic CD4 T cells were strikingly absent in the lymph nodes. CXCR5CD4 T cells, representing putative follicular T-h (Tfh) cells were over-represented in lymph nodes and expressed higher levels of Tfh markers than their peripheral blood counterparts. Compared with the circulating pool, lymph-node-derived CXCR5CD4 T cells were superior in providing help to B cells. Thus, functionally competent Tfh cells accumulate in resting human lymph nodes, providing a swift induction of naive and memory antibody responses upon antigenic challenge.