Influence of sea buckthorn (Hippophae rhamnoides L.) flavone on dermal wound healing in rats
Influence of sea buckthorn (Hippophae rhamnoides L.) flavone on dermal wound healing in rats
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DOI：
10.1007/s11010-006-9187-6
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发表时间：
2006-10-01
影响因子：
4.3
通讯作者：
Sawhney, Ramesh C.
中科院分区：
文献类型：
--
作者：
Gupta, Asheesh;Kumar, Ratan;Sawhney, Ramesh C.
本研究旨在探讨沙棘黄酮局部给药的疗效。对大鼠皮肤伤口愈合的影响。在大鼠背部创建四个全层切除伤口，并局部应用在丙二醇中制备的1.0%w/v黄酮。对照动物以相同方式单独接受溶媒。通过伤口收缩率、上皮形成时间、羟脯氨酸、氨基己糖、抗氧化剂的测定和肉芽组织的组织病理学来评估伤口的愈合。沙棘黄酮促进伤口愈合活性，如通过改善伤口收缩率、减少上皮形成所需时间（16.3天对对照组的24.8天）以及显著增加羟脯氨酸（26.0%）和己糖胺（30.0%）含量所示。这些发现也得到了组织病理学检查的证实。此外，还观察到沙棘黄酮具有有效的抗氧化特性，如伤口肉芽组织中还原型谷胱甘肽（55.0%）、维生素C（70.0%）和过氧化氢酶（20.0%）活性的显著增加所证明的。黄酮处理也导致脂质过氧化物水平显著降低（39.0%）。结果表明，沙棘黄酮促进伤口愈合活性。
The present investigation was undertaken to determine the efficacy of topical administration of flavone of sea buckthorn (Hippophae rhamnoides L.) on cutaneous wound healing in rats. Four full-thickness excision wounds were created on the back of rat and 1.0% w/v flavone prepared in propylene glycol was applied topically. Control animals received the vehicle alone in an identical manner. The healing of the wound was assessed by the rate of wound contraction, period of epithelialization, hydroxyproline, hexosamine, antioxidants estimation and histopathology of the granulation tissue. The sea buckthorn flavone promoted the wound healing activity as indicated by improved rate of wound contraction, decreased time taken for epithelialization (16.3 days versus 24.8 days in controls) and significant increase in hydroxyproline (26.0%) and hexosamine (30.0%) content. These findings were also confirmed by histopathological examinations. In addition, it was observed that sea buckthorn flavone possesses potent antioxidant properties as evidenced by significant increase in reduced glutathione (55.0%), vitamin C (70.0%) and catalase (20.0%) activities in wound granulation tissue. The flavone treatment also resulted in significant decrease in lipid peroxide levels (39.0%). The results suggest that the sea buckthorn flavone promotes wound healing activity.